Suspension cultures are more suitable for physiological, biochemical and molecular investigations than callus cultures grown on solid media, because the former provide more homogeneous system than the latter ones. A large number of plants were found suitable for establishing cell suspension cultures. In this study we describe the obtaining of cell suspension cultures derived from callus induced from cotyledons of Pharbitis nil. Explants isolated from plants grown under inductive and non-inductive conditions were cultured on MS basal medium containing various concentrations and combinations of growth regulators. To initiate the cell suspension culture, small clumps of friable callus obtained from cotyledons were suspended in liquid callusing medium. An initial inoculum density was 2104cells/ml. Every 20 days the cultures were transferred to a fresh medium. The cell number in suspension was determined by direct microscopic counting with haemocytometr. The cell suspension cultures contained both single cells and small cell aggregates.