Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

• Authors: A. Mikula
• Languages of publication: PL

Abstracts

EN

This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultrastructural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/ tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.

Keywords

EN

ACCLIMATION; COLD; CRYOPRESERVATION; DESICCATION; GENETIC STABILITY

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2008
• Issue: 2
• Pages: 41-57

Contributors

author

A. Mikula

• Document Type: REVIEW
• Publication order reference: Anna Mikula, Ogrod Botaniczny ? Centrum Zachowania Roznorodnosci Biologicznej, Polska Akademia Nauk, ul. Prawdziwka 2, 02-873 Warszawa, Poland