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Journal
2008 | 2 | 41-57
Article title

Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

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PL
Abstracts
EN
This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultrastructural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/ tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.
Journal
Year
Issue
2
Pages
41-57
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References
Document Type
REVIEW
Publication order reference
Anna Mikula, Ogrod Botaniczny ? Centrum Zachowania Roznorodnosci Biologicznej, Polska Akademia Nauk, ul. Prawdziwka 2, 02-873 Warszawa, Poland
YADDA identifier
bwmeta1.element.element-from-psjc-1436f104-b1c6-38ad-81ac-9515f29a743b
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