PL EN


Preferences help
enabled [disable] Abstract
Number of results
2005 | 46 | 2 | 217-225
Article title

DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response

Content
Title variants
Languages of publication
EN
Abstracts
EN
Arylamine N-acetyltransferase (NAT) genes were targeted for inhibition using short hairpin RNA (shRNA) using two different RNA polymerase III promoters. Constructs were developed for NAT1 and NAT2, the endogenous mouse genes, and for human NAT1. There were fetal and neonatal deaths with these constructs, perhaps due in part to an interferon response as reflected in increases in oligoadenylate synthetase I mRNA levels. Seven out of 8 founders with the U6 promoter generated offspring but only 2 gave positive offspring. Out of 15 founders for H1 promoted constructs, only 4 had positive offspring. When transgenic lines were successfully established, the expression of the targeted genes was variable between animals and was not generally inhibitory.
Discipline
Year
Volume
46
Issue
2
Pages
217-225
Physical description
References
Document Type
ARTICLE
Publication order reference
R.P. Erickson, Department of Pediatrics 4341B. 1501 N. Campbell Avenue, P.O. Box 245073, Tucson, Arizona 85724-5073
YADDA identifier
bwmeta1.element.element-from-psjc-14364255-0f48-37fc-ab1c-101a1ff00655
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.