Effects of acute and chronic Triazolam treatments on brain GABA levels in albino rats

• Authors: S.M. Aburawi , Elhuwuegi A.E. Elhuwuegi , A.E. Elhuwuegi , Ahmed S.S. Ahmed , S.S. Ahmed , Saad S.F. Saad , S.F. Saad , Attia A.S. Attia , A.S. Attia
• Languages of publication: EN

Abstracts

EN

The present study investigated the effects of acute and chronic intraperitoneal administration of Triazolam on g-aminobutyric acid (GABA) levels in different brain areas of albino rats. Three experiments were conducted. In the first, five groups of rats were acutely treated with different doses of Triazolam (0.25 mg/kg-4.0 mg/kg). In the second experiment, rats were treated chronically with a single daily dose of Triazolam (started with 0.25 mg/kg and increased by time to 1.0 mg/kg) for 5 weeks, simulating clinical use. In the third, rats were treated chronically with three daily doses of Triazolam (started with 0.25 mg/kg and increased by time to 0.5 mg/kg) for 20 days, representing a form of drug abuse. Brain levels of GABA and plasma levels of Triazolam were measured using high performance liquid chromatography (HPLC). The acute Triazolam administration produced an increase in GABA levels in all brain areas studied. The chronic administration of single daily dose of Triazolam produced normal GABA levels in all brain areas except brain stem where the levels were significantly decreased, this indicates the development of tolerance to Triazolam action on increasing GABA content. The chronic administration of three daily doses of Triazolam produced a decrease in GABA levels in all brain regions studied. In conclusion, chronic single daily dose treatment (representing normal use) produces tolerance to Triazolam effects on brain GABA levels, while chronic three daily doses administration (akin to drug abuse) causes a fall in GABA levels.

Keywords

EN

DRUG ABUSE; GABA; HPLC; RAT; TOLERANCE; TRIAZOLAM

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2000
• Volume: 60
• Issue: 4
• Pages: 447-455

Contributors

author

S.M. Aburawi

author

Elhuwuegi A.E. Elhuwuegi

author

A.E. Elhuwuegi

author

Ahmed S.S. Ahmed

author

S.S. Ahmed

author

Saad S.F. Saad

author

S.F. Saad

author

Attia A.S. Attia

author

A.S. Attia

• Document Type: ARTICLE
• Publication order reference: S. M. Aburawi, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Great Alfateh University of Medical Sciences, Tripoli, Libya