Regulation of proenkephalin gene expression by transcription factors Fos and CREB: an antisense oligonucleotide approach
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The present study investigated the effects of antisense oligonucleotides (AS ODNs) against c-fos and CREB m RNA in two models of the proenkephalin (PENK) gene induction.AS ODNs to both c-fos and CREB mRNA markedly reduced induction of the PENK gene in the rat hippocampus in vivo during seizures produced by kainic acid (KA).In contrast, in an in vitro model of the PENK gene induction by noradrenaline and dexamothasone in C6 glioma cells, the AS ODN to c-fos was without effect, whereas the AS ODN to CREB reduced the increase in the PENK mRNA level.The obtained results suggest that the transcription factors Fos and CREB may mediate induction of the PENK gene in the hippocampus, while induction of this gene in C6 glioma is mediated by CREB rather than Fos.
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R.Przewlocki, Institute of Pharmacology, Polish Academy of Sciences, Smetna St.12, 31-343 Krakow, Poland