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1998 | 39 | 2 | 193-198
Article title

Morphology and developmental potential of bovine parthenotes after spontaneous activation in vitro

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EN
Abstracts
EN
Spontaneous parthenogenetic activation of bovine oocytes in an in vitro maturation and fertilization system (IVM/IVF) is described. Altogether, 1403 follicular oocytes, collected by the aspiration method, were matured in vitro and then cultured without insemination in the same conditions as a group of inseminated oocytes. After 48-72 h of additional culture, 141 oocytes (10%) were found to be spontaneously activated. Morphological evaluation revealed that the number of blastomeres within parthenotes ranged from 2 to 16 cells, with a minority (15.7%) comprising of 9-16 blastomeres. According to a cytogenetic analysis, only 1.2% of the analysed parthenotes consisted of more than 9 cells. Parthenotes may not be distinguished from embryos produced in vitro and spontaneous parthenogenetic activation in an IVM/IVF system indicates suboptimal culture conditions. A group of non-inseminated oocytes should be included in each experiment to serve as a control. Spontaneously activated bovine parthenotes only occasionaly developed beyond the 8-blastomere stage in a common IVM/IVF system. The incidence of parthenotes interferes with the efficiency of in vitro embryo production but it is doubtful whether it lowers the pregnancy rate after transfer of IVF embryos.
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Year
Volume
39
Issue
2
Pages
193-198
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author
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D.Lechniak, Department of Genetics and Animal Breeding, Agricultural University of Poznan, ul. Wolynska 33, 60-637 Poznan, Poland
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bwmeta1.element.element-from-psjc-11f5a28f-b70c-3584-8549-3e7e44e03a7a
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