Cytological observations of mitotic chromosomes were performed for two species and interspecific hybrid lines: Lupinus hispanicus subsp. hispanicus (line Badajoz 2, cat. no. Wt 96383), L. luteus cv. Topaz (cat. no. Wt 98006), L. ? hispanicoluteus (cat. no. Wt 98301 and Wt 98302). It was found that L. hispanicus, L. luteus and L. ? hispanicoluteus have the same chromosome number 2n = 52. Chromosome morphology is, generally, similar. Centromeres are located mostly in median or submedian region. Chromosome differentiation appears to be very poor, except the first (the longest) pair which is easily discernible in both parental species and in the hybrid.