Application of flow cytometry for the study of plant genomes

• Authors: J. Dolezel
• Languages of publication: EN

Abstracts

EN

During the last fifteen years, flow cytometry and sorting greatly contributed to the improvement of our knowledge of plant genome structure and function. This paper reviews the applications of flow cytometry for the analysis of isolated nuclei and chromosomes. Because of its speed, precision and convenience, this method of analysis of the nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained with chromosome analysis and sorting indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.

Keywords

EN

DNA CONTENT; FLOW CYTOGENETICS; FLOW CYTOMETRY; FLOW KARYOTYPE; NUCLEUS; PLOIDY

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1997
• Volume: 38
• Issue: 3
• Pages: 285-302

Contributors

author

J. Dolezel

• Document Type: review article
• Publication order reference: J.Dolezel, Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovsk? 6, CZ 77200 Olomouc, Czech Republic.