Selection of strains and medium composition for Yarrowia lipolytica biomass production on lipid substrates
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The production of Single Cell Protein in the batch culture from crude rapeseed oil and waste fatty acids using Y. lipolytica strains was studied. The effect of nitrogen sources, lipid concentration and pH value on the yeast growth and biomass yield was also investigated. Yarrowia lipolytica ATCC 8661 was the best strain for SCP production on both lipid substrates. The specific growth rate and biomass yield were m = 0,19 - 0,35 h-1 and Yx/s = 0,68 - 1,07 respectively, depending on the culture condition. With the above yeast strain it was possible to carry out the fermentation at low pH 3,5 without any significant lowering of kinetic and yield parameters.
Publication order reference
W. Rymowicz, Katedra Biotechnologii i Mikrobiologii Zywnosci, Akademia Rolnicza, ul. Norwida 25, 50-375 Wroclaw, Poland