PL EN


Preferences help
enabled [disable] Abstract
Number of results
1998 | 39 | 1 | 113-127
Article title

Analysis of chromosome aberrations, sister chromatid exchanges (SCE) and cell division kinetics in human lymphocytes exposed in vitro to new monophosphates of pyrimidine acyclonucleosides

Title variants
Languages of publication
Abstracts
EN
Five newly synthesised monophosphates of two pyrimidine acyclonucleoside series, namely 1-N-[(2'-hydroxy)ethoxymethyl] and 1-N-[(1',3'-dihydroxy)-2'-propoxymethyl] derivatives of 5- and 5,6-alkylated uracils were tested in vitro for chromosome aberrations and sister chromatid exchanges (SCE). Metaphase plates were obtained via microculture of human lymphocytes from heparinized peripheral blood. The compounds were tested in doses: 10, 20, 40, 80 and 150 ?g per mL of culture. The tested compounds induced mainly chromatid gaps, less frequently chromosome gaps. A low number of mitoses with chromatid and chromosome breaks, acentric fragments, dicentric chromosomes and exchange figures were also observed. The tested compounds in doses: 40, 80 and 150 ?g per mL, doubled or tripled the percentage of cells with chromatid gaps and chromosome gaps as compared to the control. The percentage of cells with aberrations (excluding gaps) induced by the tested compounds in all doses did not exceed 2%. The tested compounds induced a higher number of SCE per cell but less than double frequency as compared to the control. SCE frequencies and replication index (RI) values varied depending on the examined compounds. For the highest dose of the tested compounds (150 ? per mL) a significant decrease in RI values was observed for 1-N-[(2'-hydroxy)ethoxymethyl]-5,6-tetramethyleneuracil monophosphate and for 1-N-[(2'-hydroxy)ethoxymethyl] -5,6-dimethyluracil monophosphate. So far, the results have indicated potential clastogenicity of all the tested compounds except acycloguanosine monophosphate.
Discipline
Publisher

Year
Volume
39
Issue
1
Pages
113-127
Physical description
Contributors
author
author
author
author
author
References
Document Type
Publication order reference
T. Ferenc, Department of Biology and Medical Genetics, Military Medical Academy, Pl. Gen. J. Hallera 1, 90-647 Lodz, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-0daf0423-84bf-3f41-b748-ce1fda43370f
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.