Transformed root cultures of Arnica montana L. ? of secondary metabolites isolation
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The hairy roots of A. montana were achieved by the infection of sterile leaves with Agrobacterium rhizogenes (strain LBA 9402). The transformation was confirmed by opin and PCR analysis. In the investigation, the optimum contitions of Hairy roots growth were characterized. The best results were achieved in liquid Gamborg medium which included half-strenght macro- and microelements (1/2B5) and 50 g/l sucrose. Phytochemical analysis showed that hairy roots of A. montana produced arnifolin and chlorogenic acid.
Publication order reference
I. Weremczuk-Jezyna, Zaklad Biologii i Botaniki Farmaceutycznej, Uniwersytet Medyczny, ul. Muszynskiego 1, 90-151 Lodz, Poland