Is parallel, intramolecular association of 5'-->3' oligoribonucleotide strand possible in RNA under physiological conditions ?

• Authors: K. Lamparska
• Languages of publication: PL

Abstracts

EN

The article briefly presents the design and chemical synthesis of parallel stranded DNA with formation of reverse Watson-Crick base pairs. Tchurikov et al attempted an analysis of parallel RNA-RNA duplex formation. Single stranded RNA, prepared by in vitro transcription with T7 polymerase using 33P and 3H nucleoside 5'-triphosphates, were appropriately hybridized and analysed by polyacrylamide gel electrophoresis prior and after RNAze A digestion. Results obtained allow to conclude on the formation of parallel stranded, RNaze A - stable RNA. The presented data show the importance of intramolecular parallel association of the single stranded RNA during its folding.

Keywords

EN

PARALLEL STRANDED DNA; PARALLEL STRANDED RNA; RNA FOLDING

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1997
• Issue: 2
• Pages: 65-70

Contributors

author

K. Lamparska

• Document Type: article
• Publication order reference: K.Lamparska, Instytut Chemii Bioorganicznej PAN, Noskowskiego 12/14, 61-704 :Poznan, Poland