Is parallel, intramolecular association of 5'-->3' oligoribonucleotide strand possible in RNA under physiological conditions ?

• Authors: K. Lamparska
• Languages of publication: PL

Abstracts

EN

The article briefly presents the design and chemical synthesis of parallel stranded DNA with formation of reverse Watson-Crick base pairs. Tchurikov et al attempted an analysis of parallel RNA-RNA duplex formation. Single stranded RNA, prepared by in vitro transcription with T7 polymerase using 33P and 3H nucleoside 5'-triphosphates, were appropriately hybridized and analysed by polyacrylamide gel electrophoresis prior and after RNAze A digestion. Results obtained allow to conclude on the formation of parallel stranded, RNaze A - stable RNA. The presented data show the importance of intramolecular parallel association of the single stranded RNA during its folding.

Keywords

EN

PARALLEL STRANDED DNA   PARALLEL STRANDED RNA   RNA FOLDING  

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1997
• Issue: 2
• Pages: 65-70
• Document Type: article
• Publication order reference: K.Lamparska, Instytut Chemii Bioorganicznej PAN, Noskowskiego 12/14, 61-704 :Poznan, Poland