Analysis of the wheat endosperm transcriptome

• Authors: D.L. Laudencia-Chingcuanco , B.S. Stamova , G.R. Lazo , X. Cui , O.D. Anderson
• Languages of publication: EN

Abstracts

EN

Among the cereals, wheat is the most widely grown geographically and is part of the staple diet in much of the world. Understanding how the cereal endosperm develops and functions will help generate better tools to manipulate grain qualities important to end-users. We used a genomics approach to identify and characterize genes that are expressed in the wheat endosperm. We analyzed the 17 949 publicly available wheat endosperm EST sequences to identify genes involved in the biological processes that occur within this tissue. Clustering and assembly of the ESTs resulted in the identification of 6 187 tentative unique genes, 2 358 of which formed contigs and 3 829 remained as singletons. A BLAST similarity search against the NCBI non-redundant sequence database revealed abundant messages for storage proteins, putative defense proteins, and proteins involved in starch and sucrose metabolism. The level of abundance of the putatively identified genes reflects the physiology of the developing endosperm. Half of the identified genes have unknown functions. Approximately 61% of the endosperm ESTs has been tentatively mapped in the hexaploid wheat genome. Using microarrays for global RNA profiling, we identified endosperm genes that are specifically up regulated in the developing grain.

Keywords

EN

ENDOSPERM; TRANSPOSON; WHEAT

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2006
• Volume: 47
• Issue: 4
• Pages: 287-302

Contributors

author

D.L. Laudencia-Chingcuanco

author

B.S. Stamova

author

G.R. Lazo

author

X. Cui

author

O.D. Anderson

• Document Type: ARTICLE
• Publication order reference: D.L. Laudencia-Chingcuanco, Western Regional Research Center, USDA-ARS 800 Buchanan Street, Albany, CA 94710, USA