Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines
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In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations.Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation.The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants).Plants obtained vie embryo callus culture showed good vegetative vigour and well-developed root system.Spike morphology of all plants resambled that of rye.Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The hetromorphic bivalent-like configurations occured in five plants in 0.01-0.02 per cell.The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with the chiasm frequency of 9.36-17.75 per cell.All plants, with 14 and 28 chromosomes, were completly sterile both in backcrosses and when selfed.
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