FISH mapping in cattle (Bos taurus L.) is not yet out of fashion

• Authors: Lorenzi L. De_ , L. Molteni , P. Parma
• Languages of publication: EN

Abstracts

EN

Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.

Keywords

EN

CATTLE; CYTOGENETIC; FLUORESCENCE; GENOME; HYBRIDIZATION

Discipline

• GENETICS: GENETICS

• Journal: Journal of Applied Genetics
• Year: 2010
• Volume: 51
• Issue: 4
• Pages: 497-499

Contributors

author

Lorenzi L. De_

author

L. Molteni

author

P. Parma

• Document Type: ARTICLE
• Publication order reference: P. Parma, Dipartimento di Scienze Animali, Via Celoria 2, 20133 Milano, Italy