PL EN


Preferences help
enabled [disable] Abstract
Number of results
2010 | 51 | 2 | 123-131
Article title

Transferability, amplification quality, and genome specificity of microsatellites in Brassica carinata and related species

Title variants
Languages of publication
EN
Abstracts
EN
No information is available on the transferability and amplification quality of microsatellite (SSR) markers of the public domain in Brassica carinata A. Braun. The objective of the presented research was to study the amplification of a set of 73 SSRs from B. nigra (L.) Koch and B. napus L. in B. carinata, and to compare the results with those obtained in the amplification of the same markers in other Brassica species of the U triangle. This set of SSRs from B. nigra (B genome) and B. napus (AC genome) allows the identification of the 3 basic genomes of the Brassica species tested. 94.3% of the SSR markers from B. nigra and 97.4% of those from B. napus amplified SSR-specific products in B. carinata. Very high-quality amplification with a strong signal and easy scoring in B. carinata was recorded for 52.8% of the specific loci from B. nigra SSRs and 59.3% of the specific loci from B. napus SSRs, compared to 66.7% in B. nigra and 62.8% in B. napus. Genome specificity and amplification quality of B. nigra and B. napus SSR markers in the 6 species under study is reported. High-quality transferable SSR markers provide an efficient and cost-effective platform to advance in molecular research in B. carinata.
Discipline
Publisher

Year
Volume
51
Issue
2
Pages
123-131
Physical description
Contributors
References
Document Type
ARTICLE
Publication order reference
B. Perez-Vich, Institute for Sustainable Agriculture (CSIC), Alameda del Obispo s/n, E-14004 Cordoba, Spain
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-08e4932c-db93-3ead-bc62-a709dc0ac005
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.