Micropropagation of Arnica montana L. and sesquiterpene lactones in regenerated plants

• Authors: I. Weremczuk-Jezyna , H. Wysokinska
• Languages of publication: PL

Abstracts

EN

Plants of Arnica montana L. were micropropagated from shoot tips. Shoot proliferation was obtained on Murashige and Skoog (MS) medium containing auxin (IAA 0.5 M/l) and cytokinin (BAP, Z, TDZ). BAP (0.25 M/l) and Z (0.5 M/l) were most effective for proliferation and elongation of shoots. Under these conditions, about 6 shoots per explant were obtained. Shoot proliferation was also achieved in the presence of TDZ (0.05 M/l). However, when used in higher concentration (0.2-10 M/l), thidiazuron caused shoot vitrification. Rooting of shoots was induced an MS medium without growth regulators. The plantlets were transplanted into pots. Phytochemical analysis showed that 4-week-old plantlets produced sesquiterpene lactone.

Keywords

EN

ARNICA MONTANA; MICROPROPAGATION; SESQUITERPENE LACTONE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2000
• Issue: 4
• Pages: 118-122

Contributors

author

I. Weremczuk-Jezyna

author

H. Wysokinska

• Document Type: ARTICLE
• Publication order reference: I. Weremczuk-Jezyna, Zaklad Biologii i Botaniki Farmaceutycznej, Akademia Medyczna, ul. Muszynskiego 1, 90-151 Lodz, Poland