Micropropagation of Arnica montana L. and sesquiterpene lactones in regenerated plants
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Plants of Arnica montana L. were micropropagated from shoot tips. Shoot proliferation was obtained on Murashige and Skoog (MS) medium containing auxin (IAA 0.5 M/l) and cytokinin (BAP, Z, TDZ). BAP (0.25 M/l) and Z (0.5 M/l) were most effective for proliferation and elongation of shoots. Under these conditions, about 6 shoots per explant were obtained. Shoot proliferation was also achieved in the presence of TDZ (0.05 M/l). However, when used in higher concentration (0.2-10 M/l), thidiazuron caused shoot vitrification. Rooting of shoots was induced an MS medium without growth regulators. The plantlets were transplanted into pots. Phytochemical analysis showed that 4-week-old plantlets produced sesquiterpene lactone.
Publication order reference
I. Weremczuk-Jezyna, Zaklad Biologii i Botaniki Farmaceutycznej, Akademia Medyczna, ul. Muszynskiego 1, 90-151 Lodz, Poland