The male karyotypes of six species of the buprestid genera Sphenoptera (two species) and Acmaeoderella (four species) were studied after Ag-banding. The following karyotypes were found in individual species: n=19-23, with predominance of 23, in Sphenoptera scovitzi Fald., 2n = 24 (22 + Xyp) in Sphenoptera mesopotamica Mars., 2n = 18 (16 + Xyr) in Acmaeoderella flavofasciata Pill. & Mitt., Acmaeoderella gibbulosa Men., Acmaeoderella boryi Brulle, Acmaeoderella vetusta Men. In Sph. scovitzi, two chromosome pairs were found to display stable argentophilous blocks. In Sph. mesopotamica, NOR-bearing is one of the large autosomal bivalents. In all species of the genus Acmaeoderella, a nucleolus-like argentophilous body is placed between the X and y chromosomes.