Propagation of Derzsy disease virus in goose embryo fibroblasts. An application of the Celligen Plus bioreactor
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Derzsy desease is a fatal hepatitis of young geese under the age of 5 weeks. The etiological agent is a goose parvovirus (GPV) which is widespread in all major goose farming countries in Europe and Asia. At present, vaccination is the only method preventing the loss of animals. In vitro GPV replicates only in goose embryo fibroblasts (GEF) and this way the virus is propagated in order to obtain the material for diagnostic tests and vaccine production. In this paper, we report the application of bioreactor Celligen Plus for GEF culture and Derzsy disease virus propagation. The method is based on immobilization and culture of cells on porous glass carriers in bioreactor. Comparing to the traditional cell culture techniques, the described method was over 12-fold more efficient and allowed to obtain good quality virus pool for Derzsy disease vaccine preparation.
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W. Rzeski, Zak?ad Wirusologii i Immunologii, Instytut Mikrobiologii i Biotechnologii, Uniwersytet Marii Curie-Sk?odowskiej, ul. Akademicka 19, 20-033 Lublin, Poland