The application of immobilized invertase for continuous production of inverted sucrose
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Four inorganic supports (sand, silica gel, crushed pumice and controlled-pore glass) were used for immobilization of invertase. The possibility of application of thus obtained immobilized enzyme in continuous production of inverted sugar was tested. The best support for immobilization of invertase was controlled-pore glass (CPG, average pore size of 31 mm). The conditions of production of inverted sugar by immobilized enzyme were optimised (also in column). Unter the optimised the column (dimensions: 0.7x27 cm) with immobilized invertase was able to degrade sucrose to glucose and fructose in an amount of 60 g/h. Immobilized invertase in the column remained fully active for 120 days.
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A. Belcarz, Zaklad Biochemii, Uniwersytet Marii Curie-Sklodowskiej, pl. M. Curie-Sklodowskiej 3, 20-031 Lublin, Poland