In vitro shoot regeneration of strawberry as the source of somaclonal variants resistant to verticillium wilt?
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Somaclonal variants of strawberry resistant to verticillium wilt were selected by cocultivation of shoots of various cultivars obtained by both micropropagation and regeneration from a callus with a homogenate of 3-week-old liquid culture of mixed isolates of Veriticillium dahliae. There was a large variation in the resistance to the pathogen within a population of both micropropagated and regenerated from the callus shoots. After 70 days of cocultivation most of the shoots of susceptible cultivars were heavily damaged or dead, but there were some individuals that survived in a relatively good health. Those variants were transplanted on a new Boxus?s medium supplemented with 2 mg/l of benomyl to eliminate pathogen and their resistance to verticillium wilt is now tested.
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I. Sowik, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland