Evaluation of competent embryo production and conversion potential in callus cultures initiated in vitro from leaf explants of six sweet potato [ipomoea batatas (l.) lam] genotypes
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The aim of this study was to evaluate in vitro embryogenic potential of selected sweet potato [Ipomoea batatas L. (Lam.)] cultivars. Leaf blade and petiole from Ipomoea batatas plants (genotypes Rojo Blanco, White Jewel, White Star, Nemanete, Papota, and IIA-TIB 10) grown in pots and in vitro cultures were used for experiments as primary explants. The potential of embryogenic callus induction and somatic embryo development was evaluated for the cultures grown on the media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), and/or abscisic acid (ABA), under different light conditions (photoperiod, constant darkness). The cultivars appeared to differ with regards to embryogenic response of explants, somatic embryogenesis progression as well as embryo development and plant regeneration potential. The highest frequency of somatic embryogenesis was found for cv. Rojo Blanco and embryo development resulted in fully regenerated, healthy plants. In other cultivars, somatic embryo induction occurred, but embryogenesis has been arrested at the globular stage. White Star appeared to be the only completely recalcitrant cultivar which did not show any embryogenic response.
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A. K. Kononowicz, Department of Cytogenetics and Plant Molecular Biology, University of Lodz, S. Banacha 12/16, 90-237 Lodz, Poland