Gelatin sponge Spongostan as a carrier for animal and human adherent cell culture

• Authors: R. Paduch , W. Rzeski , B. Zdzisinska , J. Kaczor , M. Kandefer-Szerszen
• Languages of publication: PL

Abstracts

EN

A porous gelatin sponge Spongostan (Ferrosan Denmark) as a carrier for the cultivation of several adherent cell lines and strains was tested. This material provide considerably greater surface available for cells than conventional bottles. Moreover, it allows to maintain high cell viability (over 87%) during culture time. When cells were cultured on Spongostan discs (wet vol. 0.11 cm3), no phenotypic changes in cell adherence and proliferation dynamics were observed. However, when cells adhered to Spongostan were frozen down and kept for 5 months in liquid nitrogen, they lost about 65% of their viability. Generally, Spongostan can be considered as a universal carrier for three-dimensional culture of many cell types, but it cannot be used for cells? freezing.

Keywords

EN

CELL CULTURE; GELATIN SPONGE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Journal: Biotechnologia
• Year: 2003
• Issue: 1
• Pages: 223-233

Contributors

author

R. Paduch

author

W. Rzeski

author

B. Zdzisinska

author

J. Kaczor

author

M. Kandefer-Szerszen

• Document Type: ARTICLE
• Publication order reference: R. Paduch, Zaklad Wirusologii i Immunologii, Instytut Mikrobiologii i Biotechnologii, Wydzial Biologii i Nauk o Ziemi, Uniwersytet Marii Curie-Sklodowskiej, ul. Akademicka 19, 20-033 Lublin, Poland