CIRSIUM OLERACEUM L. SCOP. INHIBITS CELL VIABILITY AND DECREASES SECRETION OF MATRIX METALLOPROTEINASES (MMP2 AND MMP9) IN GLIOBLASTOMA CELL LINE
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The aim of this study was to evaluate the activity of Cirsium oleraceum L. Scop. methanol extract (CoM) on likely glioblastoma (U87MG) and normal human astroglia (SVGp12) cell lines. CoM in concentrations 25; 50; 100; 250 and 500 µg/ml inducting significant dose- and time-dependent reductions of U87MG, as well as SVGp12 cells viability, in particular after 72 h of incubation. Activation of caspase-3 in U87MG cells suggests the pro-apoptotic activity of CoM. Moreover, CoM decreased secretion of matrix metalloproteinases (MMP2 and MMP9) and moderate decreased migration of U87MG cells in scratch test. These results suggest that Cirsium oleraceum L. Scop. methanol extract has anti-glioma effect in vitro through activation of caspase-3 and inhibition of metalloproteinase-2 and -9 in U87MG cells. Despite this, cytotoxic effect of CoM in normal human astroglia cells may limit its potential application in the adjuvant therapy of glioblastoma.
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