Molecular Fingerprints of Thyroid Cancer Cells by Using Library of Molecular Receptors Formed by N-Lipidated Peptides Immobilized on Cellulose

• Authors: Justyna Fraczyk , Małgorzata Walczak , Waldemar Balcerzak , Katarzyna Pokajewicz , Piotr Wieczorek , Wojciech Młynarski , Wojciech Fendler , Zbigniew J. Kaminski
• Languages of publication: EN

Abstracts

EN

A novel diagnostic method based on recognition of qualitative and quantitative composition of healthy and tumor tissue samples by library of molecular receptors was presented. Molecular receptors were formed by self-organization of N-lipidated peptides attached in the regular fashion via aminophenylamino-1,3,5-triazine linker to the surface of cellulose plate. For samples testing, the library was cloned into multiple, identical copies and for each experiment the new clone was used. The binding process was monitored by staining the discs with Brilliant Black and quantitative color measurement was performed in 256 grade gray scale. Substantial differences in the composition of healthy and tumor samples were observed in most cases. The highly individual chemical fingerprints were found to be reliant on the cancer type. For malignant papillary thyroid cancer statistical analysis identified two receptors most useful for diagnostic purposes.

Keywords

EN

surface modification; cancer markers; chemical receptor; molecular mono-layer; immobilized peptides

• Journal: Acta Poloniae Pharmaceutica - Drug Research
• Year: 2018
• Volume: 75
• Issue: 4
• Pages: 1017-1029

Dates

published

2018-08-31

Contributors

author

Justyna Fraczyk

author

Małgorzata Walczak

author

Waldemar Balcerzak

author

Katarzyna Pokajewicz

author

Piotr Wieczorek

author

Wojciech Młynarski

author

Wojciech Fendler

author

Zbigniew J. Kaminski

Identifiers

DOI

10.32383/appdr/78538