PL EN


Preferences help
enabled [disable] Abstract
Number of results
2020 | 77 | 1 | 3-10
Article title

ANALYTICAL STUDY AND ANTIMICROBIAL ACTIVITY OF ALPHA-DEFENSIN 2 DISSOLVED IN PHARMACOPOEIA BUFFERS WITH DIFFERENT pH

Content
Title variants
Languages of publication
EN
Abstracts
EN
Human neutrophil peptides (HNPs, alpha-defensins) are a group of six defensins being considered as new antimicrobial drugs because of their multifunctional efficiency against bacteria, viruses and fungi. Regardless of the unique biological properties alpha-defensins are unstable compounds and their activity depends on many physical and chemical factors as well as on the kind of the used cultivation media. This leads to research difficulties and obstructions in their therapeutic application. The purpose of this study was to determine antibacterial activity of alpha-defensin 2 dissolved in pharmacopeia buffers and in parallel to develop selective and accurate analytical tests for identification and assay of alpha-defensin 2 in the course of study. The antibacterial effect of alpha-defensin 2 was determined against the strain Escherichia coli (ATCC 25922). It was found that 10 mg/l of Human neutrophil peptide-2 (HNP-2, alpha-defensin 2) dissolved in pH 9.0 buffer caused 90% inhibition of the bacterial respiratory activity. This buffer was considered as a suitable environment for deploying the antibacterial activity of the alpha-defensin. А selective MS analysis method for identification of alpha-defensin 2 in sample mixtures was developed. Also HPLC method with alternative selectivity was elaborated for identification and assay of mixtures containing alpha-defensin 2 and aminoacids in Mueller Hinton broth. The procedure includes development of system suitability test determination.
Year
Volume
77
Issue
1
Pages
3-10
Physical description
Dates
published
2020-02-29
References
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.doi-10_32383_appdr_113345
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.