Biomarkery wykorzystywane w ocenie oksydacyjnych uszkodzeń białek

• Authors: Katarzyna Ognik , Ewelina Cholewińska

Title variants

EN

Biomarkers of proteins oxidative damage

• Languages of publication: PL EN

Abstracts

PL

Przewaga procesów prooksydacyjnych w organizmie skutkuje wystąpieniem stresu oksydacyjnego objawiającego się m.in. utlenianiem białek. Bezpośrednia analiza ilości reaktywnych form tlenu i azotu jest zadaniem bardzo trudnym, dlatego w ocenie nasilenia stresu oksydacyjnego częściej wykorzystuje się markery uszkodzeń, powstające w wyniku reakcji wolnych rodników z białkami. Są one o wiele trwalsze, a przez to łatwiejsze do analizy. Wśród najważniejszych biomarkerów oksydacyjnych uszkodzeń białek wyróżnia się pochodne karbonylowe, 3-nitrotyrozynę, S-nitrotriazole, kynureninę, 3-chlorotyrozynę, bromotyrozynę, sulfotlenek metioniny, dityrozynę, oksohistydynę oraz tzw. zaawansowane produkty oksydacji białek (AOPP).
W ocenie oksydacyjnych uszkodzeń białek u zwierząt laboratoryjnych najlepiej sprawdzają się pochodne karbonylowe, 3-nitrotyrozyna i AOPP. Ich zawartość w ustroju wyraźnie wzrasta w odpowiedzi na stres oksydacyjny wywołany takimi czynnikami, jak: niewłaściwa dieta, niedobór mikroelementów, zatrucie substancjami toksycznymi, infekcje czy starzenie.

EN

The prevalence of prooxidative processes in the body is associated with development of oxidative stress, one of the symptoms of which is oxidation of proteins. Direct analysis of the amount of reactive forms of oxygen and nitrogen is a very difficult task. Therefore, in assessing the severity of oxidative stress, markers generated by free radical reactions with proteins are often used. They are much more durable and thus easier to analyze. The most important biomarkers of oxidative damage of proteins are protein carbonyl compounds, 3-nitrothyrosine, S-nitrotriazoles, kynurenine, 3-chlorothyrozine, bromothyroxine, methionine sulfoxide, dithyrosine, oxohistidine and and so called advanced oxidation protein products (AOPP).
Protein carbonyls, 3-nitrotyrosine and AOPP are the best indicators for evaluating of oxidative damage of proteins in laboratory animals. Their content in the body is clearly increasing in response to oxidative stress caused by such factors as improper diet, micronutrient deficiencies, toxic poisoning, infections or aging.

Keywords

PL

białko; biomarker; 3-nitrotyrozyna; pochodne karbonylowe; stres oksydacyjny; zaawansowane produkty utleniania białek

EN

3-nitrotyrosine; advanced oxidation protein products; biomarker; oxidative stress; protein; protein carbonyl

• Journal: Kosmos
• Year: 2018
• Volume: 67
• Issue: 2
• Pages: 347-359

Dates

published

2018

Contributors

author

Katarzyna Ognik

• Katedra Biochemii i Toksykologii, Wydział Biologii, Nauk o Zwierzętach i Biogospodarki, Uniwersytet Przyrodniczy w Lublinie, Akademicka 13, 20-950 Lublin, Polska
• Department of Biochemistry and Toxicology, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, 13 Akademicka Str., 20-950 Lublin, Poland

author

Ewelina Cholewińska

• Katedra Biochemii i Toksykologii, Wydział Biologii, Nauk o Zwierzętach i Biogospodarki, Uniwersytet Przyrodniczy w Lublinie, Akademicka 13, 20-950 Lublin, Polska
• Department of Biochemistry and Toxicology, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, 13 Akademicka Str., 20-950 Lublin, Poland

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