Mikroskopowe metody badania cytoszkieletu

• Authors: Natalia Nowak , Paweł Pomorski

Title variants

EN

Microscopy techniques for cytoskeleton resarch

• Languages of publication: PL EN

Abstracts

PL

Cytoszkielet to sieć białkowych polimerów oraz związanych z nimi setek białek motorycznych, regulatorowych i łączących cytoszkielet z innymi strukturami komórkowymi. Rozwój wiedzy o cytoszkielecie jest nierozerwalnie zwiększany z postępem technik mikroskopowych używanych do jego obserwacji. Początki tych badań to niespecyficzne, nieskomplikowane barwienia utrwalonego materiału biologicznego, które później rozwinęły się w nowoczesną mikroskopię strukturalną, pozwalającą na precyzyjne znakowanie określonych białek tworzących cytoszkielet, badanie ich stanu fizjologicznego czy też oddziaływań cytoszkieletu z luźno związanymi białkami błony czy cytoplazmy. Obecnie możliwe jest nie tylko obrazowanie struktury i funkcji cytoszkieletu ze znacznie lepszą rozdzielczością przestrzenną, ale także prowadzenie tych obserwacji na żywym materiale biologicznym. Z drugiej strony, stabilność cytoszkieletu umożliwia poszukiwanie nowych metod jego obrazowania, co niewątpliwie należy do kół napędowych postępu, jaki dokonał się i wciąż dokonuje się w dziedzinie mikroskopii.

EN

Cytoskeleton is basically a network of protein polymers, but it also contains thousands of motor, regulatory and scaffolding proteins that interact with this network. Discoveries related to the cytoskeleton were strictly connected to the development of microscopy techniques used to observe the cytoskeletal structures. At first, the imaging involved only unspecific, very simple staining of fixed material. Then, the methods evolved into advanced structural microscopy, which enabled accurate detection of specific cytoskeletal proteins, their physiological status, and interactions with loosely bound membrane and cytoplasmic proteins. Today, it is possible not only to visualize the structure and function of the cytoskeleton with better spatial resolution but also to perform the imaging in vivo on live biological specimens. On the other hand, one should also notice that observations of the stable, well defined cytoskeletal structures from their very discovery have continuously stimulated the progress in the microscopy field.

Keywords

PL

cytoszkielet; immunocytochemia; mikroskopia elektronowa; mikroskopia optyczna; mikroskopia superrozdzielcza; sondy molekularne

EN

cytoskeleton; electron microscopy; immunocytochemistry; molecular probes; optical microscopy; superresolution microscopy

• Journal: Kosmos
• Year: 2018
• Volume: 67
• Issue: 1
• Pages: 219-232

Dates

published

2018

Contributors

author

Natalia Nowak

• Pracownia Obrazowania Struktury i Funkcji Tkankowych, Centrum Neurobiologii, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN, Pasteura 3, 02-093 Warszawa, Polska
• Laboratory of Imaging Tissue Structure and Function, Neurobiology Center, Nencki Institute of Experimental Biology PAS, 3 Pasteur Str., 02-093 Warsaw, Poland

author

Paweł Pomorski

• Pracownia Molekularnych Podstaw Ruchów Komórkowych, Zakład Biochemii, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN, Pasteura 3, 02-093 Warszawa, Polska
• Środowiskowe Multimodalne Laboratorium Adhezji i Ruchu Komórek, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN, Pasteura 3, 02-093 Warszawa, Polska
• Laboratory of Molecular Basis of Cell Motility, Department of Biochemistry, Nencki Institute of Experimental Biology PAS, 3 Pasteur Str., 02-093 Warsaw, Poland
• Multimodal Laboratory of Cell Adhesion and Motility, NanoBioGeo Consortium, Nencki Institute of Experimental Biology PAS, 3 Pasteur Str., 02-093 Warsaw, Poland

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