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2017 | 66 | 2 | 193-206
Article title

Metody oparte o amplifikację DNA techniką PCR wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych

Title variants
Methods based on DNA PCR-amplification for evaluation of the soil microbial diversity
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Mikroorganizmy glebowe, pod względem cech genomowych i fenotypowych, stanowią wysoce zróżnicowaną grupę organizmów żywych. Z powodu tak dużej różnorodności ważne jest dobranie odpowiednich metod, dających największy stopień różnicowania mikroorganizmów. Narzędziami umożliwiającym analizę zmienności genetycznej mikroorganizmów są techniki genetyczne, a wśród nich jedną z najważniejszych jest łańcuchowa reakcja polimerazy, czyli PCR (Polymerase Chain Reaction), technika opracowana w latach 1980. Niniejsza praca stanowi przegląd podstawowych zagadnień dotyczących badania zmienności genetycznej mikroorganizmów glebowych w oparciu o markery molekularne z wykorzystaniem technik bazujących na reakcji PCR tj. PCR-RFLP, TRFLP, ARDRA, RAPD.
Soil microorganisms represent a highly diverse group of living organisms in terms of genomic and phenotypic characteristics. Due to such a large diversity, it is important to select appropriate identification methods which would secure its most complete determination. Genetic techniques are proper tools of choice for analyzing genetic variability of microorganism, the most important of which is the polymerase chain reaction (PCR), developed in the 1980s. This work presents an overview of the basic issues concerning studies on genetic variability of soil microorganisms with help of molecular markers and application of PCR techniques such as PCR-RFLP, TRFLP, ARDRA, RAPD.
Physical description
  • Zakład Mikrobiologii Rolniczej, Instytut Uprawy Nawożenia i Gleboznawstwa, Państwowy Instytut Badawczy, Czartoryskich 8, 24-100 Puławy, Polska
  • Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland
  • Zakład Mikrobiologii Rolniczej, Instytut Uprawy Nawożenia i Gleboznawstwa, Państwowy Instytut Badawczy, Czartoryskich 8, 24-100 Puławy, Polska
  • Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland
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