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2016 | 65 | 1 | 103-115
Article title

Fluorescencja chlorofilu a - historia odkrycia i zastosowanie w badaniach roślin

Title variants
Chlorophyll a fluorescence - history of discovery and practical application in environmental plant science
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Fluorescencja chlorofilu a jest czułą, nieinwazyjną i szybką metodą pomiaru wydajności fotosystemu II (PSII). Artykuł przedstawia wprowadzenie teoretyczne, historię odkrycia fenomenu, opis najczęściej używanych technik oraz praktyczne zastosowanie pomiarów fluorescencji chlorofilu a w badaniach. Scharakteryzowano trzy główne metody pomiaru fluorescencji chlorofilu a tj. szybką, modulowaną oraz jej obrazowanie. Analiza parametrów fotoluminescencji chlorofilu a dostarcza wielu informacji o funkcjonowaniu PSII roślin rosnących w warunkach stresu abiotycznego i biotycznego, jest powszechnie wykorzystywana przez fizjologów roślin oraz ekofizjologów. Przedstawiono najnowsze wyniki badań wpływu wybranych niekorzystnych warunków środowiska (promieniowanie świetlne, wysoka temperatura, przechłodzenie, susza, zalanie, uszkodzenie mechaniczne) na zmiany parametrów fluorescencji chlorofilu a. Artykuł jest wprowadzeniem do tematyki pomiarów fluorescencji chlorofilu a i jest przeznaczony dla osób zainteresowanych wykorzystaniem jej w swoich badaniach.
Chlorophyll a fluorescence is a sensitive, non-invasive fast tool for measuring photosynthetic efficiency mainly of photosystem II (PSII). We present description of basic photoluminescence mechanism, history of chlorophyll fluorescence discovery and review of main chlorophyll fluorescence measurement techniques with practical issue. In this article, we focus on methods of a fast chlorophyll fluorescence, pulse-amplitude modulated chlorophyll fluorescence and chlorophyll fluorescence imaging technique. Described techniques are powerful and widely use tools, available for plant physiologists and ecophysiologists. Analysis of the chlorophyll fluorescence parameters, which are good indicators or biomarkers of plant tolerance, provides many information about efficiency of PSII during abiotic and biotic stress. We describe how environmental stress conditions (irradiance, heat, cold, drought, flood and mechanical wounding) influence to most popular chlorophyll a fluorescence parameters and how to interpret them. The aim of this review is to provide a simple, practical guide to chlorophyll fluorescence for beginners.
Physical description
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