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2015 | 64 | 1 | 31-45
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Proteazy serynowe i ich klasyfikacja według systemu merops

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PL
Enzymy proteolityczne inaczej nazywane także proteazami, proteinazami lub peptydazami są to enzymy należące do klasy hydrolaz, które posiadają zdolność do hydrolitycznego rozkładu wiązania peptydowego, czyli proteolizy . Enzymy te zostały zidentyfikowane zarówno w organizmach prokariotycznych jak i eukariotycznych. Wykazano, że typowy genom ludzki zawiera około 2% genów, które odpowiedzialne są za kodowanie enzymów proteolitycznych.
PL
Proteazy serynowe stanowią prawie jedną trzecią wszystkich enzymów hydrolizujących wiązanie peptydowe. Nazwa tych proteaz pochodzi od obecności nukleofilowego aminokwasu seryny, znajdującej się w miejscu aktywnym enzymu, która atakuje grupę karbonylową wiązania peptydowego tworząc produkt pośredni reakcji tzw. acylo-enzym. Ze względu na to, że reakcje katalizowane przez proteazy są bardzo złożone, Rawlings i Barrett zaproponowali bardzo precyzyjny system opisujący klasyfikacje enzymów proteolitycznych - MEROPS. Według tego systemu proteazy są podzielone na klany, w których następnie wyróżnione są rodziny. Najnowsza baza MEROPS podzieliła proteazy serynowe na 15 klanów, w których znajdują się łącznie 53 rodzin. Celem tej pracy jest krótka charakterystyka proteaz serynowych oraz ich podział na poszczególne klany.
EN
Proteolytic enzymes, known also as the proteases, proteinases or peptidases, belong to the class of hydrolases involved in hydrolytic degradation of peptide bonds. These enzymes have been identified in both prokaryotic and eukaryotic cells. Typical human genome contains about 2% of the genes that are responsible for encoding of proteolytic enzymes. Serine proteases represent almost one-third of all proteolytic enzymes. The name of these proteases is derived from the presence of the nucleophilic amino acid: serine located in the active site of the enzyme, which attacks the carbonyl group of the peptide bond forming thereby an intermediate called acyl-enzyme. Due to the fact that the reactions catalyzed by proteases are very complex Rawlings and Barrett proposed a high precision classification system for proteolytic enzymes called MEROPS. Under this system, proteases are divided into clans and families. The recent MEROPS database divided serine proteases into 15 clans containing a together 53 families. The aim of this paper is all short description of serine proteases and their division into different clans.
Keywords
Journal
Year
Volume
64
Issue
1
Pages
31-45
Physical description
Dates
published
2015
Contributors
author
  • Katedra Biochemii Ogólne, Wydział Biologii i Ochrony Środowiska, Uniwersytet Łódzki, Pomorska 141/143, 90-236 Łódź, Polska
  • Katedra Biochemii Ogólne, Wydział Biologii i Ochrony Środowiska, Uniwersytet Łódzki, Pomorska 141/143, 90-236 Łódź, Polska
author
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