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2013 | 62 | 2 | 221-233
Article title

Ilościowa analiza obrazu w badaniach strukturalnej plastyczności synaptycznej

Title variants
Quantitative image analysis in the structural synaptic plasticity studies
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Analiza obrazów mikroskopowych odgrywa obecnie dominującą rolę w badaniach nad strukturą mózgu. Wgląd w strukturalną plastyczność synaptyczną może być kluczem do zrozumienia podstaw wielu zaburzeń neurodegeneracyjnych. Prawie w każdym eksperymencie niezbędna jest ilościowa analiza obrazów tkanki mózgowej, wymagająca często wyspecjalizowanego oprogramowania komputerowego ze względu na złożoność struktur analizowanych obrazów. W niniejszym tekście dokonamy przeglądu najważniejszych problemów towarzyszących analizie obrazów zebranych mikroskopem konfokalnym. Każdy z tych problemów wymaga zastosowania dedykowanych algorytmów.
The analysis of confocal microscopy images has started to play a significant role in the brain structure analysis. An insight into structural synaptic plasticity may be the key to elucidate the molecular basis of many neurodegenerative disorders. Almost every experiment demands a quantitative analysis of brain tissue images, which requires specialized software able to cope with the structural complexity of the data. In the following text we will review common issues arising while performing the confocal image analysis and dedicated algorithms designed to overcome those problems.
Physical description
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  • Pracownia Obrazowania Struktury i Funkcji Tkanek, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska
  • Pracownia Biofizyki Komórki, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska
  • Pracownia Neuromorfologii Molekularnej i Systemowej, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska
  • Pracownia Neuromorfologii Molekularnej i Systemowej, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska
  • Pracownia Biofizyki Komórki, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska
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