Mikroskopowe techniki korelacji fluorescencji

• Authors: Tytus Bernaś

Title variants

EN

Fluorescence correlation microscopy

• Languages of publication: PL EN

Abstracts

PL

Ruchliwość molekuł ma fundamentalne znaczenie w wielu procesach fizycznych, chemicznych oraz biologicznych. Mikroskopowe pomiary z użyciem korelacji zmian (fluktuacji) fluorescencji pozwalają wyznaczyć współczynniki dyfuzji, stałe wiązania oraz stężenia znakowanych molekuł. Niniejszy artykuł opisuje podstawy pomiarów punktowych przy użyciu korelacji fluorescencji w czasie (FCS) oraz pochodnych metod wykorzystujących przestrzenną korelację fluorescencyjnego obrazu mikroskopowego i korelację czasowo-przestrzenną.

EN

Molecular mobility is crucial in numerous physical, chemical and biological processes. Microscopy measurements of fluorescence changes (fluctuations) can be used to estimate diffusion coefficients, binding constants and concentrations of labeled molecules. This short review describes the principles of point-wise microscopio measurements with fluorescence correlation spectroscopy. Furthermore, basics of its derivatives using spatial and spatio-temporal correlation of fluorescence microscopy data are given.

• Journal: Kosmos
• Year: 2013
• Volume: 62
• Issue: 2
• Pages: 205-211

Dates

published

2013

Contributors

author

Tytus Bernaś

• Pracownia Obrazowania Struktury i Funkcji Tkanek Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska

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