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2013 | 62 | 2 | 205-211
Article title

Mikroskopowe techniki korelacji fluorescencji

Title variants
Fluorescence correlation microscopy
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Ruchliwość molekuł ma fundamentalne znaczenie w wielu procesach fizycznych, chemicznych oraz biologicznych. Mikroskopowe pomiary z użyciem korelacji zmian (fluktuacji) fluorescencji pozwalają wyznaczyć współczynniki dyfuzji, stałe wiązania oraz stężenia znakowanych molekuł. Niniejszy artykuł opisuje podstawy pomiarów punktowych przy użyciu korelacji fluorescencji w czasie (FCS) oraz pochodnych metod wykorzystujących przestrzenną korelację fluorescencyjnego obrazu mikroskopowego i korelację czasowo-przestrzenną.
Molecular mobility is crucial in numerous physical, chemical and biological processes. Microscopy measurements of fluorescence changes (fluctuations) can be used to estimate diffusion coefficients, binding constants and concentrations of labeled molecules. This short review describes the principles of point-wise microscopio measurements with fluorescence correlation spectroscopy. Furthermore, basics of its derivatives using spatial and spatio-temporal correlation of fluorescence microscopy data are given.
Physical description
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