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Journal
2013 | 62 | 2 | 205-211
Article title

Mikroskopowe techniki korelacji fluorescencji

Authors
Content
Title variants
EN
Fluorescence correlation microscopy
Languages of publication
PL EN
Abstracts
PL
Ruchliwość molekuł ma fundamentalne znaczenie w wielu procesach fizycznych, chemicznych oraz biologicznych. Mikroskopowe pomiary z użyciem korelacji zmian (fluktuacji) fluorescencji pozwalają wyznaczyć współczynniki dyfuzji, stałe wiązania oraz stężenia znakowanych molekuł. Niniejszy artykuł opisuje podstawy pomiarów punktowych przy użyciu korelacji fluorescencji w czasie (FCS) oraz pochodnych metod wykorzystujących przestrzenną korelację fluorescencyjnego obrazu mikroskopowego i korelację czasowo-przestrzenną.
EN
Molecular mobility is crucial in numerous physical, chemical and biological processes. Microscopy measurements of fluorescence changes (fluctuations) can be used to estimate diffusion coefficients, binding constants and concentrations of labeled molecules. This short review describes the principles of point-wise microscopio measurements with fluorescence correlation spectroscopy. Furthermore, basics of its derivatives using spatial and spatio-temporal correlation of fluorescence microscopy data are given.
Keywords
Journal
Year
Volume
62
Issue
2
Pages
205-211
Physical description
Dates
published
2013
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-ksv62p205kz
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