Ruch pod mikroskopem - współczesne techniki badania adhezji i ruchliwości komórek

• Authors: Paweł Pomorski

Title variants

EN

Motion under the microscope - modern techniques for studying cell adhesion and motility

• Languages of publication: PL EN

Abstracts

PL

Ruch komórek jest jedną z ich fundamentalnych cech. To dzięki niemu organizm może się rozwijać, układ odpornościowy działać, organy regenerować, a rany goić się. Badanie mechanizmów ruchu jest jednak jedną z trudniejszych metodycznie dziedzin biologii komórki. Procesy biochemicznie zachodzą bowiem w ruchliwych komórkach niesynchronicznie, a często takich komórek jest niewiele. Niniejszy artykuł poświęcono metodom mikroskopowym stworzonym by rozwiązać ten problem. Omawiane są metody pomiaru parametrów ruchu i adhezji do podłoża. Omawiane są również podstawy zastosowania klasycznych metod mikroskopowych do badania procesów ruchowych. Pokrótce wspominamy też o wykorzystaniu sond molekularnych do badania procesów sygnalizacji w komórkach ruchliwych, a także o mikroskopowych metodach eksperymentalnych, umożliwiających doświadczenia na pojedynczych komórkach.

EN

Ability to move is one of the fundamental functions of the living cells. It is due to the motility that organism develops, immune system can work, organs are able to regenerate and wound heal. In the same time motility studies are among methodologically most difficult ones. Biochemical processes underlying motility are notoriously unsynchronized and motile cells are usually not very numerous. Current paper reviews microscope techniques developed to solve those problems. We discuss basic measurements, parameterizing motility and substratum adhesion. Classical, structural microscopy used for motility studies are also sketched shortly. We describe also use of molecular probes for signaling studies in motile cells as well as we mention about microscopic experimental techniques, allowing experiments on single cells.

• Journal: Kosmos
• Year: 2013
• Volume: 62
• Issue: 2
• Pages: 181-192

Dates

published

2013

Contributors

author

Paweł Pomorski

• Zakład Biochemii Instytut Biologii Doświadczalnej im. M. Nenckiego PAN Pasteura 3, 02-093 Warszawa, Polska

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