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Article title

Budowa przestrzenna i działanie rybosomu bakteryjnego. nagroda nobla z chemii 2009

Title variants
The structure and function of the ribosome. Nobel Prize in chemistry 2009
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The Nobel prize in chemistry was awarded to Venkatraman Ramakrishnan, Thomas A. Steitz and Ada E. Yonath for their studies on the structure and function of the ribosome. These scientists used X-ray crystallography to determine the three dimensional structure of the bacterial ribosome at atomic resolution. The knowledge of the positions of atoms in such a large macromolecular complex helped to resolve its mechanism of action. Their studies confirmed that the ribosome is a ribozyme and exerts its catalytic power from the RNA and not proteins. This paves way to design novel antibiotics targeting the bacterial ribosome. Next step would be to determine a high resolution structure of the eukaryotic ribosome which is larger and more complicated than the prokaryotic one.
Physical description
  • Interdyscyplinarne Centrum Modelowania Matematycznego i Komputerowego, Pawińskiego 5A, 02-106 Warszawa, Polska
  • Agmon F., Bashan A., Zarivach R., Yonath A., 2005. Symmetry at the active site of the ribosome: Structural and functional implications. Biol. Chem. 386, 833-844.
  • Appelt K., Dijk J., Reinhardt R., Sanhuesa S., White S. W., Wilson K. S., Yonath A., 1981. The crystallization of ribosomal-proteins from the 50S subunit of the escherichia-coli and bacillus-stearothermophilus ribosome. J. Biol. Chemi. 256, 1787-1790.
  • Bąkowska-Żywicka K., Twardowski T., 2008. Struktura i funkcjonowanie rybosomu eukariotycznego. Post. Biochem. 54, 251-263.
  • Ban N., Nissen P., Hansen J., Moore P. B., Steitz T. A., 2000. The complete atomic structure of the large ribosomal subunit at 2.4 angstrom resolution. Science 289, 905-920.
  • Bokov K., Steinberg S. V., 2009. A hierarchical model for evolution of 23S ribosomal rna. Nature 457, 977-980.
  • Borovinskaya M. A., Shoji S., Fredrick K., Cate J. H. D., 2008. Structural basis for hygromycin b inhibition of protein biosynthesis. Rna-a Publication of the Rna Society 14, 1590-1599.
  • Carter A. P., Clemons W. M., Brodersen D. E., Morgan-Warren R. J., Wimberly B. T., Ramakrishnan V., 2000. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature 407, 340-348.
  • Carter A. P., Clemons W. M., Brodersen D. E., Morgan-Warren R. J., Hartsch T., Wimberly B. T., Ramakrishnan V., 2001. Crystal structure of an initiation factor bound to the 30S ribosomal subunit. Science 291, 498-501.
  • Cate J. H., Yusupov M. M., Yusupova G. Z., Earnest T. N., Noller H. F., 1999. X-ray crystal structures of 70S ribosome functional complexes. Science 285, 2095-2104.
  • Clemons W. M., May J. L. C., Wimberly B. T., Mccutcheon J. P., Capel M. S., Ramakrishnan V., 1999. Structure of a bacterial 30S ribosomal subunit at 5.5 angstrom resolution. Nature 400, 833-840.
  • Frank J., Zhu J., Penczek P., Li Y. H., Srivastava S., Verschoor A., Radermacher M., Grassucci R., Lata R. K., Agrawal R. K., 1995. A model of protein-synthesis based on cryoelectron microscopy of the e-coli ribosome. Nature 376, 441-444.
  • Gao Y. G., Selmer M., Dunham C. M., Weixlbaumer A., Kelley A. C., Ramakrishnan V., 2009. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 326, 694-699.
  • Harms J., Schluenzen F., Zarivach R., Bashan A., Gat S., Agmon I., Bartels H., Franceschi F., Yonath A., 2001. High resolution structure of the large ribosomal subunit from a mesophilic eubacterium. Cell 107, 679-688.
  • Markiewicz Z., Kwiatkowski Z. A., 2006. Bakterie, antybiotyki, lekooporność, PWN, Warszawa.
  • Pioletti M., Schlunzen F., Harms J., Zarivach R., Gluhmann M., Avila H., Bashan A., Bartels H., Auerbach T., Jacobi C., Hartsch T., Yonath A., Franceschi F., 2001. Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3. EMBO J. 20, 1829-1839.
  • Rhodes, G., 2006. Crystallography made crystal clear. Elsevier Inc.
  • Rodnina M. V., Wintermeyer W., 1998. Form follows function: Structure of an elongation factor G-ribosome complex. Proc. Natl. Acad. Sci. USA 95, 7237-7239.
  • Rodnina M. V., Beringer M., Wintermeyer W., 2007. How ribosomes make peptide bonds. Trends Biochem. Sci. 32, 20-26.
  • Schluenzen F., Tocilj A., Zarivach R., Harms J., Gluehmann M., Janell D., Bashan A., Bartels H., Agmon I., Franceschi F., Yonath A., 2000. Structure of functionally activated small ribosomal subunit at 3.3 angstrom resolution. Cell 102, 615-623.
  • Schlunzen F., Zarivach R., Harms J., Bashan A., Tocilj A., Albrecht R., Yonath A., Franceschi F., 2001. Structural basis for the interaction of antibiotics with the peptidyl transferase centre in eubacteria. Nature 413, 814-821.
  • Schmeing T. M., Huang K. S., Kitchen D. E., Strobel S. A., Steitz T. A., 2005. Structural insights into the roles of water and the 2'-hydroxyl of the p site tRNA in the peptidyl transferase reaction. Mol. Cell 20, 437-448.
  • Schmeing T. M., Voorhees R. M., Kelley A. C., Gao Y. G., Murphy F. V., Weir J. R., Ramakrishnan V., 2009. The crystal structure of the ribosome bound to EF-Tu and aminoacyl-trna. Science 326, 688-694.
  • Schuwirth B. S., Borovinskaya M. A., Hau C. W., Zhang W., Vila-Sanjurjo A., Holton J. M., Cate J. H. D., 2005. Structures of the bacterial ribosome at 3.5 angstrom resolution. Science 310, 827-834.
  • Shevack A., Gewitz H. S., Hennemann B., Yonath A., Wittmann H. G., 1985. Characterization and crystallization of ribosomal particles from halobacterium-marismortui. FEBS Lett. 184, 68-71.
  • Tocilj A., Schlunzen F., Janell D., Gluhmann M., Hansen H. A. S., Harms J., Bashan A., Bartels H., Agmon I., Franceschi F., Yonath A., 1999. The small ribosomal subunit from thermus thermophilus at 4.5 angstrom resolution: Pattern fittings and the identification of a functional site. Proc. Natl. Acad. Sci. USA 96, 14252-14257.
  • Vonbohlen K., Makowski I., Hansen H. A. S., Bartels H., Berkovitchyellin Z., Zaytzevbashan A., Meyer S., Paulke C., Franceschi F., Yonath A., 1991. Characterization and preliminary attempts for derivatization of crystals of large ribosomal-subunits from haloarcula-marismortui diffracting to 3-a resolution. J. Mol. Biol. 222, 11-15.
  • Weixlbaumer A., Jin H., Neubauer C., Voorhees R. M., Petry S., Kelley A. C., Ramakrishnan V., 2008. Insights into translational termination from the structure of RF2 bound to the ribosome. Science 322, 953-956.
  • Yusupov M. M., Yusupova G. Z., Baucom A., Lieberman K., Earnest T. N., Cate J. H. D., Noller H. F., 2001. Crystal structure of the ribosome at 5.5 angstrom resolution. Science 292, 883-896.
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Publication order reference
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