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Journal

2004 | 53 | 3-4 | 295-303

Article title

Mikromacierze DNA

Content

Title variants

EN
DNA microarrays

Languages of publication

PL EN

Abstracts

EN
Summary The breakthrough in DNA sequencing technology and completion of sequencing of first eucaryotic genomes raised the need for high-throughput methods of gene function analysis. To solve this problem the DNA microarray technology has been developed. It is based on traditional transcript profiling methods which use the hybridization ability of DNA and RNA to monitor gene expression. Due to miniaturisation and the use of fluorescent dyes DNA microarrays allow for simultaneous monitoring of the expression of tens of thousands genes. There are two main types of DNA microarrays: cDNA microarrays and oligonucleotide microarrays, also called DNA chips. In the former, several- hundred-nucleotide long cDNA probes are printed on a glass plate and hybridized to a fluorescently labeled target cDNA obtained from the tissue of interest. In the latter, type of microarrays, each gene is represented by several short oligonucleotides (about 30 nt), perfectly matching the target gene, and several oligomers with a single mismatch. Oligonucleotides are usually synthetised on a glass slide using the photolithographic technique and the slide is hybridized to fluorescently labeled target RNA. Analysis ofmicroarray data includes the comparison of gene expression in the experimental and control probes or the comparison of gene expression profiles obtained in several experiments, by different clustering methods. Medicine is one of the fields where DNA microarrays have already found practical application. At present they are especially useful in cancer research. DNA microarray analysis of tumor tissues allows to differentiate among various cancer types, to prognose the illness progress and plan the therapy. DNA microarrays are also an irreplaceable tool in a search for new drugs.

Keywords

Journal

Year

Volume

53

Issue

3-4

Pages

295-303

Physical description

Dates

published
2004

Contributors

  • Instytut Chemii Bioorganicznej PAN, Noskowskiego 12/14, 61-704 Poznań, Polska
author
  • Instytut Chemii Bioorganicznej PAN, Noskowskiego 12/14, 61-704 Poznań, Polska
  • Instytut Chemii Bioorganicznej PAN, Noskowskiego 12/14, 61-704 Poznań, Polska
  • Instytut Chemii Bioorganicznej PAN, Noskowskiego 12/14, 61-704 Poznań, Polska

References

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  • BROWN P. O., BOTSTEIN D., 1999. Exploring the new world of the genome with DNA microarrays. Nat. Genet. Suppl. 21, 33-37.
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  • EISEN M. B., SPELLMAN P. T., BROWN P. O., BOTSTEIN D., 1998. Cluster analysis and display of genomewide expression patterns. Proc. Natl. Acad. Sci. USA 95, 14863-14868.
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  • NAGAHATA T., ONDA M., EMI M., NAGAI H., TSUMAGARI K., FUJIMOTO T., HIRANO A., SATO T., NISHIKAWA K., AKIYAMA F., SAKAMOTO G., KASUMI F., MIKI Y., TANAKA T., TSUNODA T. 2004. Expression profiling to predict postoperative prognosis for estrogen receptor-negative breast cancers by analysis of 25,344 genes on a cDNAmicroarray. Cancer Sci. 95, 218-225.
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  • VRANA K. E., FREEMAN W. M., ASCHNER M., 2003. Use of Microarray Technologies in Toxicology Research. Neuro Toxicol. 24, 321-332.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-ksv53p295kz
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