PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2002 | 51 | 3 | 297-304
Article title

Mechanizmy stabilnego dziedziczenia plazmidów

Content
Title variants
EN
Mechanisms of plasmid stable inheritance
Languages of publication
PL EN
Abstracts
EN
Summary The stable inheritance of bacterial plasmids is achieved by a number of different mechanisms. Among them are: resolution of plasmid multimers into monomers, selective killing of plasmid-free segregants and active plasmid partitioning into dividing cells. The first two mechanisms are discussed in this article. The multimer resolution systems (mrs) consist of a site specific recombinase (resolvase) and the defined nucleotide sequence res located on the plasmid. By specific recombination between repeated res sequences the recombinase resolves plasmid oligomers to monomers. This maximizes the number of plasmid units prior to cell division and considerably contributes to stable maintenance of plasmid in bacterial cells. The post-segregational killing systems involve a stable poison and an unstable antidote. The antidotes nautralize their cognate poisons or prevent their synthesis. The different decay rates of the poisons and the antidotes underlie the molecular mechanisms of poison activation in plasmid-free cells. By killing and eliminating plasmid-free cells from the population of plasmidbearing ones the poison-antidote couples act therefore as plasmid addiction systems. While the mrs maximize the random plasmid distribution into the dividing cells addiction systems assure better-than-random plasmid distribution.
Keywords
Journal
Year
Volume
51
Issue
3
Pages
297-304
Physical description
Dates
published
2002
Contributors
  • Zakład Biochemii Drobnoustrojów Instytut Biochemii i Biofizyki PAN, Pawińskiego 5a, 02-106 Warszawa, Polska
  • Zakład Biochemii Drobnoustrojów Instytut Biochemii i Biofizyki PAN, Pawińskiego 5a, 02-106 Warszawa, Polska
References
  • AIZENMAN E., ENGELBERG-KULKA H., GLASER G., 1996. An Escherichia coli chromosomal 'addiction module' regulated by guanosine 3',5'-bispyrophosphate: a model for programmed bacterial cell death. Proc. Natl. Acad. Sci. USA 93, 6059-6063.
  • ALONSO J. C., WEISE F., ROJO F., 1995. The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035. J. Biol. Chem. 270, 2938-2945.
  • AUSTIN S., ZIESE M., STERNBERG N., 1981. A novel role for site-specific recombination in maintenance of bacterial replicons. Cell 25, 729-736.
  • COUTURIER M., BAHASSI E., VAN MELDEREN L., 1998. Bacterial death by DNA gyrase poisoning. Trends Microbiol. 6, 269-275.
  • DE LA HOZ A. B., AYORA S., SITKIEWICZ I., FERNANDEZ S., PANKIEWICZ R., ALONSO J. C., CEGŁOWSKI P., 2000. Plasmid copy-number control and better-thanrandom segregation genes of pSM19035 share a common regulator. Proc. Natl. Acad. Sci. USA 97, 728-733.
  • GERDES K., 2000. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress. J. Bacteriol. 182, 561-572.
  • GOTFREDSEN M., GERDES K. 1998. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Mol. Microbiol. 29, 1065-1076.
  • GREENFIELD T. J., EHLI E., KIRSHENMANN T., FRANCH T., GERDES K., WEAVER K. E., 2000. The antisense RNA of the par locus of pAD1 regulates the expression of a 33-amino-acid toxic peptide by an unusual mechanism. Mol. Microbiol. 37, 652-660.
  • GRINTER N. J., BREWSTER G., BARTH P. T., 1989. Two mechanisms necessary for the stable inheritance of plasmid RP4. Plasmid 22, 203-214.
  • JAFFE A., OGURA T., HIRAGA S., 1985. Effects of the ccd function of the F plasmid on bacterial growth. J. Bacteriol. 163, 841-849.
  • JIANG Y., POGLIANO J., HELINKI D.R., KONIECZNY I., 2002. ParE toxin encoded by the broad-host-range plazmid RK2 is an inhibitor of Escherichia coli gyrase. Mol. Microbiol. 44, 971-979.
  • KOBAYASHI I., 1998. Selfishness and death: raison d'etre of restriction, recombination and mitochondria. Trends Genet. 14, 368-374.
  • KOYAMA A. H., WADA C., NAGATA T., YURA T., 1975. Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli. J. Bacteriol. 122, 73-79.
  • LORIS R., DAO-THI M. H., BAHASSI E. M., VAN MELDEREN L., POORTMANS F., LIDDINGTON R., COUTURIER M., WYNS L., 1999. Crystal structure of CcdB, a topoisomerase poison from E. coli. J. Mol. Biol. 285, 167-1677.
  • NAITO T., KUSANO K., KOBAYASHI I., 1995. Selfish behavior of restriction-modification systems. Science 267, 897-899.
  • NORDSTROM K., AUSTIN S. J., 1989. Mechanisms that contribute to the stable segregation of plasmids. Annu. Rev. Genet. 23, 37-69.
  • OGURA T., HIRAGA S., 1983. Mini-F plasmid genes that couple host cell division to plasmid proliferation. Proc. Natl. Acad. Sci. USA 80, 4784-4788.
  • RUIZ-ECHEVARRIA M. J., GIMENEZ-GALLEGO G., SABARIEGOSJARENO R., DIAZ-OREJAS R., 1995. Kid, a small protein of the parD stability system of plasmid R1, is an inhibitor of DNA replication acting at the initiation of DNA synthesis. J. Mol. Biol. 247, 568-577.
  • SUMMERS D. K., 1998. Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol. Microbiol. 29, 1137-1145.
  • SUMMERS D. K., BENTON W. H., WITHERS H. L., 1993. Multicopy plasmid instability: the dimer catastrophe hypothesis. Mol. Microbiol. 8, 1031-1038.
  • SUMMERS, D. K., SHERRATT D. J., 1984. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability. Cell 36, 1097-1103.
  • THISTED T., SORENSEN N. S., GERDES K., 1995. Mechanism of Post-segregational Killing: Secondary Structure Analysis of the Entire Hok mRNA from Plasmid R1 Suggests a Fold-back Structure that Prevents Translation and Antisense RNA Binding. J. Mol. Biol. 247, 1960-1968.
  • WEAVER K. E., JENSEN K. D., COLWELL A., SRIRAM S. I., 1996. Functional analysis of the Enterococcus faecalis plasmid pAD1-encoded stability determinant par. Mol. Microbiol. 20, 53-63.
  • YARMOLINSKY M. B., 1995. Programmed cell death in bacterial populations. Science 267, 836-837.
  • ZIELENKIEWICZ U., CEGŁOWSKI P., 2001. Mechanisms of plasmid stable maintenance with special focus on plasmid addiction systems. Acta Biochim. Polon. 48, 1003-1023.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-ksv51p297kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.