Impact of Plesiomonas Shigelloides Strain CNCTC 144/92 Lipopolysaccharide on DPPC Liposome Membranes: EPR Method

• Authors: D. Man , B. Pytel , I. Pisarek
• Languages of publication: EN

Abstracts

EN

Membrane fluidity measurements were performed for synthetic DPPC liposomes sonicated in aqueous solution and doped by Plesiomonas shigelloides strain CNCTC 144/92 (serotype O74:H5) lipopolysaccharide (LPS) extracted from the phenol (LPS_{PhOH}) and water (LPS_{H₂O}) phases. Concentrations of LPS in relation to DPPC ranged from 0 to 1.4% (molar ratio). The EPR spin probe method was used to describe physicochemical properties of different regions of the lipid bilayer. Since TEMPO spin probe dissolves both in the hydrophobic region of the membrane and in an aquatic environment it is possible to determine the spectroscopic partition parameter F, indicating the changes that occur in the water-lipid interface. The 16-DOXYL probe distributed in the middle of the lipid bilayer makes it possible to obtain the rotational correlation time τ parameter, which provides information about fluidity changes in the liposome membrane. Here we report that increasing concentrations (mainly in the range of 0.4-0.8%) of investigated LPS_{PhOH} and LPS_{H₂O} significantly influence spectroscopic parameters (F and τ). The surface area of the DPPC liposomes membranes was affected predominantly by LPS_{H₂O} while the lipid bilayer was most influenced by LPS_{PhOH}.

Keywords

EN

87.16.Dg; 87.64.kh; 87.80.Lg

Discipline

• 87.80.Lg: Magnetic and paramagnetic resonance
• 87.64.kh: EPR

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2017
• Volume: 132
• Issue: 1
• Pages: 77-80

Dates

published

2017-07

Contributors

author

D. Man

• Institute of Physics, Opole University, Oleska 48, 45-052 Opole, Poland

author

B. Pytel

• Institute of Physics, Opole University, Oleska 48, 45-052 Opole, Poland

author

I. Pisarek

• Department of Land Protection, Opole University, 45-052 Opole, Oleska 22, Poland

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Identifiers

DOI

10.12693/APhysPolA.132.77