Visualization of the Interface between Cell and Ceramic Coating by Electron Microscopy and Tomography Techniques

• Authors: J. Karbowniczek , A. Gruszczyński , A. Kruk , A. Czyrska-Filemonowicz
• Languages of publication: EN

Abstracts

EN

To improve the stability of bone-material connection various surface modifications are applied to titanium based implants. Such new biomaterials require detailed characterization, including their biocompatibility. In the present study focused ion beam-scanning electron microscope tomography was applied to investigate the internal structure of ceramic coating as well as cell-material interface. Focused ion beam-scanning electron microscope tomography involves repeated focused ion beam milling and subsequent scanning electron microscope imaging of exposed sample surface and is followed by 3D reconstruction. This approach enables receiving additional information about the sample, unavailable at 2D images. The qualitative and quantitative investigation of pores in the ceramic coating was performed. The porosity of investigated volume: 8.5× 1.1× 3.9 μm³ was calculated as 24%. Additionally, based on 3D reconstruction the visualization of the coherent interface between cell and ceramic coating was done.

Keywords

EN

68.37.Hk; 81.65.Mq; 87.17.Rt; 87.85.J-

Discipline

• 87.85.J-: Biomaterials
• 87.17.Rt: Cell adhesion and cell mechanics
• 81.65.Mq: Oxidation(see also 64.75.Lm Phase separation and segregation in oxidation)
• 68.37.Hk: Scanning electron microscopy (SEM) (including EBIC)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2017
• Volume: 131
• Issue: 5
• Pages: 1357-1360

Dates

published

2017-05

Contributors

author

J. Karbowniczek

• International Centre of Electron Microscopy for Materials Science and Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, Al. A. Mickiewicza 30, PL-30059 Krakow, Poland

author

A. Gruszczyński

• International Centre of Electron Microscopy for Materials Science and Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, Al. A. Mickiewicza 30, PL-30059 Krakow, Poland

author

A. Kruk

• International Centre of Electron Microscopy for Materials Science and Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, Al. A. Mickiewicza 30, PL-30059 Krakow, Poland

author

A. Czyrska-Filemonowicz

• International Centre of Electron Microscopy for Materials Science and Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, Al. A. Mickiewicza 30, PL-30059 Krakow, Poland

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Identifiers

DOI

10.12693/APhysPolA.131.1357