Effects of Gamma Ray Irradiation and NaCl on Induced Somaclonal Variation in Arnavutköy Strawberry Cultivar

• Authors: K. Kepenek
• Languages of publication: EN

Abstracts

EN

Salinity stress impacts the growth and development of strawberry crop significantly in tropical and temperate regions across the world developing somaclonal variant which offers an option to develop salt stress tolerant genotypes. Somaclonal variants of Arnavutköy strawberry cultivar were produced via organogenesis by irradiating axillary shoots with gamma rays (20 and 40 Gy with ⁶⁰Co) along with imposing different NaCl concentrations (0, 250, 500, and 750 ppm). Increasing total doses of ⁶⁰Co together with NaCl concentrations significantly affected the percentage of survival rate, axillary shoots proliferation, length of axillary shoots, fresh weight of axillary shoots, leaf deformation scores and necrotic area of leaves. The variants with less deformation were selected for strawberry breeding. A gamma irradiation dose of 40 Gy was found to be the most effective dose together with 500 ppm of NaCl for to obtain the somaclonal variants without having much lethal effects and deformations of the strawberry breeding in vitro condition. If successfully applied, this methodology can lead to the identification of new strawberry cultivars resistant to NaCl within a relatively short period of time.

Keywords

EN

61.80.Ed

Discipline

• 61.80.Ed: γ-ray effects

• Journal: Acta Physica Polonica A
• Year: 2016
• Volume: 130
• Issue: 1
• Pages: 337-341

Dates

published

2016-07

Contributors

author

K. Kepenek

• Agricultural Biotechnology Department, Faculty of Agriculture, Suleyman Demirel University, 32260, Isparta, Turkey

References

• [1] FAO, Food and Agricultural Organization, accessed on 3 April 2014, 2012 http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567#ancor
• [2] F. Wu, G. Zhengfei, A. Whidden, Strawberry Industry Overview and Outlook, accessed on 3 April 2014, 2012 http://www.fred.ifas.ufl.edu/pdf/webinar/Strawberry.pdf
• [3] E. Werker, H.R. Lerner, R. Weimberg, A. Poljakoff-Mayber, Am. J. Botany 70, 222 (1983), doi: 10.2307/2443267
• [4] B. Bhagwat, E.J. Duncan, Euphytica 101, 143 (1998), doi: 10.1023/A:1018391619986
• [5] S.M. Jain, Euphytica 118, 153 (2001), doi: 10.1023/A:1004124519479
• [6] S.M. Jain, J. Plant Cell Tiss. Organ Cult. 82, 113 (2005), doi: 10.1007/s11240-004-7095-6
• [7] S. Vezzulli, L. Leonardelli, U. Malossini, M. Stefanini, R. Velasco, C. Moser, J. Exp. Botany 63, 6359 (2013)
• [8] F. Walther, in: Induced Mutations in Plants. Proc. IAEA/FAO Symp. on the Nature, Induction and Utilization of Mutations in Plants, Pullman (USA), IAEA/FAO, Vienna 1969, p. 261 http://trove.nla.gov.au/work/10286250?selectedversion=NBD204552
• [9] S.M. Kaeppler, H.F. Kaeppler, Y. Rhee, Plant Mol. Biol. 43, 179 (2000), doi: 10.1023/A:1006423110134
• [10] J.S. Mohan, Roman. Biotechnol. Lett. 15, 88 (2010)
• [11] S.S. Bidabadi, S. Meon, Z. Wahab, S. Subramaniam, M. Mahmood, Austral. J. Crop Sci. 6, 391 (2012)
• [12] T.B. Dao, P.D. Nguyen, Q.M. Do, T.H. Vu, T.L. Le, T.K.L. Nguyen, H.D. Nguyen, X.L. Nguyen, Plant Mutat. Rep. 1, 26 (2006)
• [13] S. Predieri, J. Plant Cell Tiss. Organ Cult. 64, 185 (2001), doi: 10.1023/A:1010623203554
• [14] S.M. Jain, Acta Horti. 447, 69 (1997)
• [15] Gu Di-Zhou, Jun-Yi Zhu, Yun-Tian Jiang, Ying Feng, Yu Liang, Cunying Ba, Li-Na Zhang, South China Fruits 2010, 15 (2010)
• [16] E.M. Linsmaier, F. Skoog, Physiol. Plant. 18, 100 (1965), doi: 10.1111/j.1399-3054.1965.tb06874.x
• [17] P. Donini, A. Sonnino, in: Somaclonal Variation and Induced Mutations in Crop Improvement, Eds. S.M. Jain, D.S. Brar, B.S. Ahloowalia, Kluwer Academic Publ., London 1998, p. 255
• [18] N. Delhomez, Hortic. Sci. 30, 592 (1995)
• [19] W. Weimin, Z. Mizhen, W. Zhuangwei, Q. Yaming, Y. Ji, ISHS Acta Horti. 842, 597 (2009), doi: 10.17660/ActaHortic.2009.842.127
• [20] GIMP, GNU Image Manipulation Program (created by S. Kimball and P. Mattis), ver. 2.2.10, 2005
• [21] Liu Yan-Meng, Xue-Ying Zhang, Hui-Bo Ge, Li-Jin Zheng, Chuan Wu, J. Agricult. Univ. Hebei 2008, 26 (2008)

Identifiers

DOI

10.12693/APhysPolA.130.337