Methodology for FTIR Imaging of Individual Cells

• Authors: Seydou Yao , M. Cestelli Guidi , M. Delugin , G. Della-Ventura , A. Marcelli , C. Petibois
• Languages of publication: EN

Abstracts

EN

FTIR imaging is a novel spectroscopic technique able to provide cell imaging, in vivo and in real-time. However, one key issue is developing methodologies for cell culture on IR-transparent substrates fitting cell biology requirements. In this work we tested different IR-transparent substrates in terms of biotoxicity, surface properties, and spectral image acquisition qualities. Only a few substrates, namely Si₃N₄, Ge, GLS, LaF₃, Si, SrF₂, ZnS/C, ZnS/F, were found to provide cell culture conditions comparable to those observed on usual polycarbonate Petri dishes, the main limiting parameter being the toxicity of the material (ZnS, GLS, PbF₂, PbCl₂) or a poor adhesiveness (notably diamond, AgCl, CaF₂, ZnS). From substrates eligible for a good-quality cell culture, the spectral acquisition quality is mainly affected by the refractive index value. Finally, the best compromise between cell culture quality and image spectral quality could be obtained using Si and Ge substrates. This rationalization of the available IR-transparent substrates for bioimaging is particularly relevant for live cell analyses, where cell culture conditions must remain unaffected by substrate properties.

Keywords

EN

82.80.-d; 87.85.Pq; 87.64.-t

Discipline

• 87.64.-t: Spectroscopic and microscopic techniques in biophysics and medical physics
• 82.80.-d: Chemical analysis and related physical methods of analysis(for related instrumentation, see section 07; for spectroscopic techniques in biological physics, see 87.64.-t)
• 87.85.Pq: Biomedical imaging

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2016
• Volume: 129
• Issue: 2
• Pages: 250-254

Dates

published

2016-02

Contributors

author

Seydou Yao

• Centre for NanoHealth, Institute of Life Science 2 Building, Swansea University, Singleton Park, Swansea, Abertawe, SA2 8PP, UK

author

M. Cestelli Guidi

• INFN, Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati (Rome), Italy

author

M. Delugin

• Université de Bordeaux; Inserm U1029 LAMC, Allée Geoffroy Saint-Hillaire, Bat B2, 33600 Pessac, France

author

G. Della-Ventura

• Université de Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint-Hillaire, Bat B2, 33600 Pessac, France

author

A. Marcelli

• INFN, Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati (Rome), Italy
• Dipartimento Scienze Geologiche, Università Roma Tre, Roma, Italy

author

C. Petibois

• Université de Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint-Hillaire, Bat B2, 33600 Pessac, France

References

• [1] C. Petibois, Anal. Bioanal. Chem. 397, 2031 (2010), doi: 10.1007/s00216-010-3731-7
• [2] A. Marcelli, A. Cricenti, W.M. Kwiatek, C. Petibois, Biotechnol. Adv. 30, 1390 (2012), doi: 10.1016/j.biotechadv.2012.02.012
• [3] N. Blow, Nature 456, 825 (2008), doi: 10.1038/456825a
• [4] F.L. Martin, J.G. Kelly, V. Llabjani, P.L. Martin-Hirsch, I.I. Patel, J. Trevisan, N.J. Fullwood, M.J. Walsh, Nat. Protoc. 5, 1748 (2010), doi: 10.1038/nprot.2010.133
• [5] S.W. Hell, Science 316, 1153 (2007), doi: 10.1126/science.1137395
• [6] Z. Liu, X. Li, S.M. Tabakman, K. Jiang, S. Fan, H. Dai, J. Am. Chem. Soc. 130, 13540 (2008), doi: 10.1021/ja806242t
• [7] S. Yao, H.H. Chen, E. Harte, G.D. Ventura, C. Petibois, Anal. Bioanal. Chem. 405, 8737 (2013), doi: 10.1007/s00216-013-7143-3
• [8] C. Petibois, M. Cestelli Guidi, Anal. Bioanal. Chem. 391, 1599 (2008), doi: 10.1007/s00216-008-2157-y
• [9] C. Petibois, B. Desbat, Trends Biotechnol. 28, 495 (2010), doi: 10.1016/j.tibtech.2010.07.003
• [10] C. Petibois, G. Déléris, Trends Biotechnol. 24, 455 (2006), doi: 10.1016/j.tibtech.2006.08.005
• [11] C. Petibois, M. Cestelli Guidi, M. Piccinini, M. Moenner, A. Marcelli, Anal. Bioanal. Chem. 397, 2123 (2010), doi: 10.1007/s00216-010-3817-2
• [12] B. Drogat, M. Bouchecareilh, C. Petibois, G. Déléris, E. Chevet, A. Bikfalvi, M. Moenner, J. Cell. Physiol. 212, 463 (2007), doi: 10.1002/jcp.21044
• [13] C. Petibois, G. Cazorla, H. Gin, G. Deleris, J. Lab. Clin. Med. 137, 184 (2001), doi: 10.1067/mlc.2001.112758
• [14] C. Petibois, G. Deleris, M. Piccinini, M. Cestelli Guidi, A. Marcelli, Nat. Photon. 3, 179 (2009), doi: 10.1038/nphoton.2009.31
• [15] M. Cestelli Guidi, S. Yao, D. Sali, S. Castano, A. Marcelli, C. Petibois, Biotechnol. Adv. 31, 402 (2013), doi: 10.1016/j.biotechadv.2011.11.009
• [16] N. Jamin, P. Dumas, J. Moncuit, W.H. Fridman, J.L. Teillaud, G.L. Carr, G.P. Williams, Proc. Natl. Acad. Sci. USA 95, 4837 (1998)
• [17] K. Wehbe, J. Filik, M.D. Frogley, G. Cinque, Anal. Bioanal. Chem. 405, 1311 (2013), doi: 10.1007/s00216-012-6521-6
• [18] C. Petibois, M. Piccinini, M. Cestelli-Guidi, A. Marcelli, J. Synchrotron Rad. 17, 1 (2010), doi: 10.1107/S0909049509043167
• [19] P. Innocenzi, T. Kidchob, J.M. Bertolo, M. Piccinini, M.C. Guidi, C. Marcelli, J. Phys. Chem. B Condens Matter. Mater. Surf. Interfaces Biophys. 110, 10837 (2006), doi: 10.1021/jp060097x
• [20] M. Diem, M. Romeo, S. Boydston-White, M. Miljkovic, C. Matthäus, Analyst 129, 880 (2004), doi: 10.1039/B408952A
• [21] H.Y. Holman, R. Miles, Z. Hao, E. Wozei, L.M. Anderson, H. Yang, Anal. Chem. 81, 8564 (2009), doi: 10.1021/ac9015424
• [22] H.-Y.N. Holman, M.C. Martin, E.A. Blakely, K. Bjornstad, W.R. Mckinney, Biopolymers 57, 329 (2000), doi: 10.1002/1097-0282(2000)57:6<329::AID-BIP20>3.0.CO;2-2
• [23] J. Kneipp, L.M. Miller, S. Spassov, F. Sokolowski, P. Lasch, M. Beekes, D. Naumann, Biopolymers 74, 163 (2004), doi: 10.1002/bip.20064
• [24] C. Paluszkiewicz, W.M. Kwiatek, A. Banas, A. Kisiel, A. Marcelli, M. Piccinini, Vib. Spectrosc. 43, 237 (2007), doi: 10.1016/j.vibspec.2006.08.005
• [25] B.R. Wood, M.A. Quinn, B. Tait, M. Ashdown, T. Hislop, M. Romeo, D. McNaughton, Biospectroscopy 4, 75 (1998), doi: 10.1002/(SICI)1520-6343(1998)4:2<75::AID-BSPY1>3.0.CO;2-R
• [26] C. Petibois, A. Cassaigne, H. Gin, G. Deleris, J. Clin. Endocrinol. Metab. 89, 3377 (2004), doi: 10.1210/jc.2003-031311

Identifiers

DOI

10.12693/APhysPolA.129.250