Application of HPLC-MS with Electrospray Ionization for the Qualitative and Quantitative Analysis of Antibiotics in Pharmaceutical Formulation

• Authors: A. Likhtarovich , V. Luhin , O. Sovastei , P. Zukowski , M. Dado
• Languages of publication: EN

Abstracts

EN

This study describes the application of electrospray ionization mass spectrometry in order to identify ten antibiotics (macrolides, penicillins, aminoglycosides). An optimum procedure was developed for determination of antibiotics of different grade. Positive ion spectra of most antibiotics are higher in intensity including an [M+H]⁺ ion and produce less fragmentation and are more informative compared to the negative ion spectra. The group of antibiotics exhibits the same characteristic fragmentation. The data base was developed for identification of antibiotics comparing of their molecular and fragment ions. The results of the study showed that the method with electrospray ionization is simple and quick which is useful in the routine determination of antibiotics and in their pharmaceutical dosage forms.

Keywords

EN

32.10.Bi; 33.15.Ta; 82.80.-d

Discipline

• 82.80.-d: Chemical analysis and related physical methods of analysis(for related instrumentation, see section 07; for spectroscopic techniques in biological physics, see 87.64.-t)
• 33.15.Ta: Mass spectra
• 32.10.Bi: Atomic masses, mass spectra, abundances, and isotopes(for mass spectroscopy, see 07.75.+h in instruments, and 82.80.Ms, Nj, Rt in physical chemistry and chemical physics)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2015
• Volume: 128
• Issue: 5
• Pages: 901-904

Dates

published

2015-11

Contributors

author

A. Likhtarovich

• Belarusian State University of Technology, 13a, Sverdlova Str., 220050 Minsk, Republic of Belarus

author

V. Luhin

• Belarusian State University of Technology, 13a, Sverdlova Str., 220050 Minsk, Republic of Belarus

author

O. Sovastei

• Belarusian State University of Technology, 13a, Sverdlova Str., 220050 Minsk, Republic of Belarus

author

P. Zukowski

• Lublin University of Technology, Nadbystrzycka 38a, 20-618 Lublin, Poland

author

M. Dado

• University of Zilina, 1, Univerzitná Str., 01026 Žilina, Slovakia

References

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Identifiers

DOI

10.12693/APhysPolA.128.901