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2015 | 128 | 2B | B-381-B-383
Article title

Cytotoxic Effects of Different ICG Concentrations and Laser Parameters on Neuroblastoma

Content
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EN
Abstracts
EN
Photodynamic therapy (PDT) is a minimally invasive treatment for cancer therapy. It can be administered in combination with other treatments such as chemotherapy, radiotherapy, and surgical excision. PDT involves a photosensitizing agent that is activated by exposure to a specific wavelength of light. PDT is a cold photochemical process, there is no tissue heating. In our study, we investigated whether different laser parameters with different concentrations of indocyanine green (ICG) have cytotoxic and anti-proliferative effects on neuroblastoma. Plates were divided groups as control, only ICG concentrations (25 and 50 μg/ml), only laser treatment I (50 J/cm²), only laser treatment II (100 J/cm²), 25 μg/ml ICG + laser treatment I and 25 μg/ml ICG + laser treatment II, 50 μg/ml ICG + laser treatment I and 50 μg/ml ICG + laser treatment II. Neuroblastoma cell lines were irradiated with an in-house developed diode laser system (λ=809 nm, 70 mW/cm², 50 & 100 J/cm²) in continuous wave operation mode after ICG application. Cell proliferation was measured by XTT assay after light irradiation. Cell proliferation was decreased in a dose-dependent manner in 25 and 50 μg/ml ICG concentrations when compared with control. The applied ICG concentrations (especially 50 μg/ml) had cytotoxic effects for neuroblastoma cell lines, SH-SY5Y. There was no difference between laser treatment groups (L 50 & 100 J/cm²). However, PDT groups (laser exposure with ICG) showed significant inhibition of cell viability (p<0.05). Additionally, laser exposure did not increase the well temperature above the incubation parameter. In conclusion, PDT has cytotoxic effects in neuroblastoma cell lines. Appropriate ICG dose - laser parameter combinations must be determined for each cell type. Different energy densities may cause different effects of PDT on inhibition of cell viability.
Keywords
EN
Publisher

Year
Volume
128
Issue
2B
Pages
B-381-B-383
Physical description
Dates
published
2015-8
Contributors
author
  • Erzincan University, Biomedical Engineering Department, Erzincan, Turkey
author
  • Boğaziçi University, Institute of Biomedical Engineering, İstanbul, Turkey
author
  • Eskişehir Osmangazi University, Medical Biology, Eskişehir, Turkey
author
  • Boğaziçi University, Institute of Biomedical Engineering, İstanbul, Turkey
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-appv128n2b113kz
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