Controlling Structural and Functional Features of Photosynthetic Antenna

• Authors: L. Fiedor , J. Fiedor , M. Pilch , A. Susz , J. Tworzydło , M. Michalik
• Languages of publication: EN

Abstracts

EN

In order to gain control over the assembly and functioning of photosynthetic antenna, we have developed methods to manipulate pigment composition of bacterial LH1 complexes via their reconstitution with modified bacteriochlorophylls or carotenoids, major photoactive cofactors of these antennae. In the present work we show how the reconstitution and pigment exchange approach is applied to control structural and functional parameters of LH1 and its subunits. The size of the subunits and the energy of the first excited singlet state can be controlled via the use of detergent while the thermodynamics of LH1 formation can be modified using carotenoids and/or a co-solvent. Carotenoids affect the efficiency of the intracomplex energy transfer, while the replacement of native bacteriochlorophyll a with its Ni-substituted analog allows one to control the excited state properties of LH1. These results show that LH1 is a very promising model system applicable for the design of bio-inspired device performing solar energy conversion.

Keywords

EN

87.15.nr; 87.15.Cc; 87.64.ks

Discipline

• 87.64.ks: Electron and photoelectron
• 87.15.nr: Aggregation
• 87.15.Cc: Folding: thermodynamics, statistical mechanics, models, and pathways(see also 87.15.hm Folding dynamics)

• Journal: Acta Physica Polonica A
• Year: 2012
• Volume: 122
• Issue: 2
• Pages: 255-258

Dates

published

2012-08

Contributors

author

L. Fiedor

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland

author

J. Fiedor

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland
• AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Kraków, Poland

author

M. Pilch

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland

author

A. Susz

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland

author

J. Tworzydło

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland

author

M. Michalik

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland

References

• 1. C.M. Davis, P.S. Parkes-Loach, C.K. Cook, K.A. Meadows, M. Bandilla, H. Scheer, P.A. Loach, Biochemistry 35, 3072 (1996)
• 2. C.M. Davis, P.L. Bustamante, J.B. Todd, P.S. Parkes-Loach, P. McGlynn, J.D. Olsen, L. McMaster, C.N. Hunter, P.A. Loach, Biochemistry 36, 3671 (1997)
• 3. R.J. Pugh, P. McGlynn, M.R. Jones, C.N. Hunter, Biochim. Biophys. Acta 1366, 301 (1998)
• 4. C. Jungas, J.-L. Ranck, J.-L. Rigaud, P. Joliot, A. Vermeglio, EMBO J. 18, 534 (1999)
• 5. R.N. Frese, J.D. Olsen, R. Branvall, W.H.J. Westerhuis, C.N. Hunter, R. van Grondelle, Proc. Natl. Acad. Sci. USA 97, 5197 (2000)
• 6. C.M. Davis, P.L. Bustamante, P.A. Loach, J. Biol. Chem. 270, 5793 (1995)
• 7. L. Fiedor, A.A. Gorman, I. Hamblett, V. Rosenbach-Belkin, Y. Salomon, A. Scherz, I. Tregub, Photochem. Photobiol. 58, 506 (1993)
• 8. J. Fiedor, L. Fiedor, J. Winkler, A. Scherz, H. Scheer, Photochem. Photobiol. 74, 64 (2001)
• 9. Carotenoids in Photosynthesis, Eds. A. Young, G. Britton, Chapmann & Hall, London 1993
• 10. S. Karrasch, P.A. Bullough, R. Ghosh, EMBO J. 14, 631 (1995)
• 11. C. Francke, J. Amesz, Photosynth. Res. 46, 347 (1995)
• 12. A.W. Roszak, T.D. Howard, J. Southall, A.T. Gardiner, C.J. Law, N.W. Isaacs, R.J. Cogdell, Science 302, 1969 (2003)
• 13. P.S. Parkes-Loach, A.P. Majeed, C.J. Law, P.A. Loach, Biochemistry 43, 7003 (2004)
• 14. A. Scherz, V. Rosenbach-Belkin, J. Fisher, Proc. Natl. Acad. Sci. USA 87, 5430 (1990)
• 15. J.A. Leegwater, J. Phys. Chem. 100, 14403 (1996)
• 16. M. Chachisvilis, O. Kühn, T. Pullerits, V. Sundström, J. Phys. Chem. 101, 7275 (1997)
• 17. J.N. Sturgis, B. Robert, J. Mol. Biol. 238, 445 (1994)
• 18. P. Braun, L. Fiedor, in The Purple Phototrophic Bacteria, Eds. C.N. Hunter, F. Daldal, M.C. Thurnauer, J.T. Beatty, Springer, Dordrecht 2009, p. 913
• 19. R. Ghosh, H. Hauser, R. Bachofen, Biochemistry 27, 1004 (1988)
• 20. M.C. Chang, P.M. Callahan, P.S. Parkes-Loach, T.M. Cotton, P.A. Loach, Biochemistry 29, 421 (1990)
• 21. V. Arluison, J. Seguin, B. Robert, Biochemistry 41, 11812 (2002)
• 22. A. Pandit, I.H.M. van Stokkum, S. Georgakopoulu, G. van der Zwan, R. van Grondelle, Photosynth. Res. 75, 235 (2003)
• 23. J. Fiedor, M. Pilch, L. Fiedor, J. Phys. Chem. B 113, 12831 (2009)
• 24. A.P. Vegh, B. Robert, FEBS Lett. 528, 222 (2002)
• 25. Z.-Y. Wang, Y. Muraoka, M. Shimonaga, M. Kobayashi, T. Nozawa, J. Am. Chem. Soc. 124, 1072 (2002)
• 26. A. Moskalenko, O. Toropygina, N. Kuznetsova, Z. Naturforsch. 51, 309 (1996)
• 27. L. Fiedor, H. Scheer, J. Biol. Chem. 280, 20921 (2005)
• 28. L. Fiedor, J. Akahane, Y. Koyama, Biochemistry 43, 16487 (2004)
• 29. J. Akahane, F.S. Rondonuwu, L. Fiedor, Y. Watanabe, Y. Koyama, Chem. Phys. Lett. 393, 184 (2004)
• 30. L. Fiedor, H. Scheer, C.N. Hunter, F. Tschirschwitz, B. Voigt, J. Ehlert, E. Nibbering, D. Leupold, T. Elsaesser, Chem. Phys. Lett. 319, 145 (2000)
• 31. L. Fiedor, D. Leupold, K. Teuchner, B. Voigt, C.N. Hunter, A. Scherz, H. Scheer, Biochemistry 40, 3737 (2001)
• 32. T. Omata, N. Murata, Plant Cell Physiol. 24, 1093 (1983)
• 33. L. Fiedor, V. Rosenbach-Belkin, A. Scherz, J. Biol. Chem. 267, 22043 (1992)
• 34. G. Hartwich, L. Fiedor, I. Simonin, E. Cmiel, W. Schäfer, D. Noy, A. Scherz, H. Scheer, J. Am. Chem. Soc. 120, 3675 (1998)
• 35. J.-P. Zhang, H. Nagae, P. Qian, L. Limantara, R. Fujii, Y. Watanabe, Y. Koyama, J. Phys. Chem. B 105, 7312 (2001)

Identifiers

DOI

10.12693/APhysPolA.122.255