Implementation of NSOM to Biological Samples

• Authors: S. Prauzner-Bechcicki , J. Wiltowska-Zuber , A. Budkowski , M. Lekka , J. Rysz
• Languages of publication: EN

Abstracts

EN

Near-field scanning optical microscopy is a technique providing images of structures with spatial resolution better than λ/2, which is undetectable in far-field where the Abbe law of limiting resolution is critical. In parallel to the optical imaging, topography maps are also acquired. Near-field scanning optical microscopy measurements can be performed both in air and liquid environments. The later makes the technique very useful for biomaterials analysis offering information that could not be obtained with other methods. Our work presents the results of recent studies on application of near-field scanning optical microscopy to imaging of cells in air as well as in physiological buffers. Differences in cell's topography and morphology have been noticed between two cell lines from human bladder non-malignant (HCV29) and malignant (T24) cancers. Presented results are part of the research that characterizes physiological changes of cells depending on stage of cancer.

Keywords

EN

87.64.M-; 87.64.mt

Discipline

• 87.64.M-: Optical microscopy
• 87.64.mt: Near-field scanning

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2012
• Volume: 121
• Issue: 2
• Pages: 533-538

Dates

published

2012-02

Contributors

author

S. Prauzner-Bechcicki

• The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, E. Radzikowskiego 152, 31-342 Kraków, Poland
• The Smoluchowski Institute of Physics, Jagiellonian University, W.S. Reymonta 4, 30-054 Kraków, Poland

author

J. Wiltowska-Zuber

• The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, E. Radzikowskiego 152, 31-342 Kraków, Poland

author

A. Budkowski

• The Smoluchowski Institute of Physics, Jagiellonian University, W.S. Reymonta 4, 30-054 Kraków, Poland

author

M. Lekka

• The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, E. Radzikowskiego 152, 31-342 Kraków, Poland

author

J. Rysz

• The Smoluchowski Institute of Physics, Jagiellonian University, W.S. Reymonta 4, 30-054 Kraków, Poland

References

• [1] O. Mooren, E. Erickson, N. Dickenson, R. Dunn, J. Lab. Autom., JALA 11, 268 (2006)
• [2] R.C. Dunn, Chem. Rev. 99, 2891 (1999)
• [3] J. Tersoff, D.R. Hamann, Phys. Rev. B 31, 805 (1985)
• [4] G. Binnig, C.F. Quate, Ch. Gerber, Phys. Rev. Lett. 56, 930 (1986)
• [5] Y. Martin, H.K. Wickramasinghe, Appl. Phys. Lett. 50, 1455 (1987)
• [6] M. Nonnenmacher, M.P. O'Boyle, H.K. Wickramasinghe, Appl. Phys. Lett. 58, 2921 (1991)
• [7] E. Betzig, J.K. Trautman, T.D. Harris, J.S. Weiner, R.L. Kostelak, Science 251, 1468 (1991)
• [8] T. Berdyyeva, C. Woodwarth, I. Sokolov, Ultramicroscopy 102, 189 (2005)
• [9] D. Allison, N. Mortensen, C. Sullivan, M. Doktycz, WIREs Nanomedicine and Nanobiotechnology 2, 618 (2010)
• [10] G. Longo, M. Girasole, G. Pompeo, R. Generosi, M. Luce, A. Cricenti, Phys. Status Solidi B 247, 2051 (2010)
• [11] M.F. Garcia-Parajó, B.I. de Bakker, M. Koopman, A. Cambi, F. de Lange, C.G. Figdor, N.F. van Hulst, Nanobiotechnol. 1, 113 (2005)
• [12] A.P. Sommer, R.P. Franke, J. Proteome Res. 1, 111 (2002)
• [13] T.H. Keller, T. Rayment, D. Klenerman, R.J. Stephenson, Rev. Sci. Instrum. 68, 1448 (1997)
• [14] C. Hoppener, J.P. Siebrasse, R. Peters, U. Kubitscheck, A. Naber, Biophys. J. 88, 3681 (2005)
• [15] M. De Serio, R. Zenobi, V. Deckert, Trends Anal. Chem. 22, 70 (2003)

Identifiers

DOI

10.12693/APhysPolA.121.533