Iron in Alzheimer's and Control Hippocampi - Mössbauer, Atomic Absorption and ELISA Studies

• Authors: J. Gałązka-Friedman , E. Bauminger , K. Szlachta , D. Koziorowski , R. Tomasiuk , A. Jaklewicz , Z. Wszołek , D. Dickson , K. Kaplińska , A. Friedman
• Languages of publication: EN

Abstracts

EN

Alzheimer disease is a neurodegenerative process of unknown mechanism taking place in a part of the brain - hippocampus. Oxidative stress and the role of iron in it is one of the suggested mechanisms of cells death. In this study several methods were used to assess iron and iron binding compounds in human hippocampus tissues. Mössbauer spectroscopy was used for identification of the iron binding compound and determination of total iron concentration in 12 control and one Alzheimer disease sample of hippocampus. Mössbauer parameters obtained for all samples suggest that most of the iron is ferritin-like iron. The average concentration of iron determined by Mössbauer spectroscopy in control hippocampus was 45±10 ng/mg wet tissue. The average concentration of iron in 10 Alzheimer disease samples determined by atomic absorption was 66±13 ng/mg wet tissue. The concentration of H and L chains of ferritin in 20 control and 10 AD hippocampi was assessed with enzyme-linked immuno-absorbent assay. The concentration of H and L ferritin was higher in Alzheimer disease compared to control (19.36±1.51 vs. 5.84±0.55 ng/μg protein for H, and 1.39±0.25 vs. 0.55±0.10 for L). This 3-fold increase of the concentration of ferritin is accompanied by a small increase of the total iron concentration.

Keywords

EN

87.64.kx

Discipline

• 87.64.kx: Mössbauer

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2011
• Volume: 119
• Issue: 1
• Pages: 81-83

Dates

published

2011-01

Contributors

author

J. Gałązka-Friedman

• Faculty of Physics, Warsaw University of Technology, Warsaw, Poland

author

E. Bauminger

• Racah Institute of Physics, Hebrew University of Jerusalem, Jerusalem, Israel

author

K. Szlachta

• Faculty of Physics, Warsaw University of Technology, Warsaw, Poland

author

D. Koziorowski

• Department of Neurology, Medical University in Warsaw, Warsaw, Poland

author

R. Tomasiuk

• Biochemistry Laboratory, Brodno Voivodship Hospital, Warsaw, Poland

author

A. Jaklewicz

• Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland

author

Z. Wszołek

• Department of Neurology, Mayo Clinic, Jacksonville, USA

author

D. Dickson

• Department of Neuroscience, Mayo Clinic, Jacksonville, USA

author

K. Kaplińska

• Faculty of Pharmacy, Medical University of Lodz, Łódź, Poland

author

A. Friedman

• Department of Neurology, Medical University in Warsaw, Warsaw, Poland

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Identifiers

DOI

10.12693/APhysPolA.119.81